Sampling and retention efficiencies of batch-type liquid-based bioaerosol samplers

Jana Kesavan, Deborah Schepers, Andrew R. McFarland

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


Four commercially available batch-type bioaerosol samplers, which collect time-integrated samples in liquids, were evaluated. Sampling efficiency was characterized as a function of particle size using near-monodisperse polystyrene spheres (sizes of 1-5 μm) and oleic acid droplets (3-10 μm). Results show the sampling efficiency of AGI-30 impingers range from 4-67% for particle sizes of 1 to 5.1 μm with significant variations between units; those of SKC BioSampler impingers range from 34-105% for particle sizes from 1 to 9 μm; those of a batch-type wetted wall cyclone with compensation for evaporation (BWWC-EC) range from 5 to 65% for particle sizes 1 to 10 μm; and, those of a batch-type wetted wall cyclone with no evaporation compensation (BWWC-NC) range of 55 to 88% for particle sizes of 1-8 μm. Retention efficiency was measured for 1 and 10 μm polystyrene spheres. For the AGI-30 and BWWC-EC, the retention efficiency of 1 μm particles after 1 h was less than 30%, while that of the SKC BioSampler was 59%. Due to liquid evaporation, the BWWC-NC could not be operated for 1 h. Retention efficiencies for Bacillus atrophaeus spores and Pantoea agglomerans vegetative cells were measured for the AGI-30 and the SKC BioSampler. Results for the spores were about the same as those for 1 μm non-viable polystyrene particles; however, the vegetative bacteria lose culturability and consequently show lower retention efficiencies. For the impingers, significant performance differences were observed in units delivered by vendors at different times.

Original languageEnglish (US)
Pages (from-to)817-829
Number of pages13
JournalAerosol Science and Technology
Issue number10
StatePublished - Oct 2010

ASJC Scopus subject areas

  • Environmental Chemistry
  • Materials Science(all)
  • Pollution


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