TY - JOUR
T1 - Sall1 transiently marks undifferentiated heart precursors and regulates their fate
AU - Morita, Yuika
AU - Andersen, Peter
AU - Hotta, Akitsu
AU - Tsukahara, Yuko
AU - Sasagawa, Noriko
AU - Hayashida, Naoko
AU - Koga, Chizuko
AU - Nishikawa, Misato
AU - Saga, Yumiko
AU - Evans, Sylvia M.
AU - Koshiba-Takeuchi, Kazuko
AU - Nishinakamura, Ryuichi
AU - Yoshida, Yoshinori
AU - Kwon, Chulan
AU - Takeuchi, Jun K.
N1 - Funding Information:
This study was supported by JST PRESTO , Grants-in-Aid for Scientific Research (B), JSPS to J.K.T., and AMED to J.K.T., A.K., Y.Y., a research fellowship for young scientist from JSPS (DC2) to Y.M. C.K. and P.A. were supported by NHLBI/NIH , MSCRF , and AHA grants.
Publisher Copyright:
© 2016 The Authors.
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Cardiac progenitor cells (CPCs) are a crucial source of cells in cardiac development and regeneration. However, reported CPCs are heterogeneous, and no gene has been identified to transiently mark undifferentiated CPCs throughout heart development. Here we show that Spalt-like gene 1 (Sall1), a zing-finger transcription factor, is expressed in undifferentiated CPCs giving rise to both left and right ventricles. Sall1 was transiently expressed in precardiac mesoderm contributing to the first heart field (left ventricle precursors) but not in the field itself. Similarly, Sall1 expression was maintained in the second heart field (outflow tract/right ventricle precursors) but not in cardiac cells. In vitro, high levels of Sall1 at mesodermal stages enhanced cardiomyogenesis, whereas its continued expression suppressed cardiac differentiation. Our study demonstrates that Sall1 marks CPCs in an undifferentiated state and regulates cardiac differentiation. These findings provide fundamental insights into CPC maintenance, which can be instrumental for CPC-based regenerative medicine.
AB - Cardiac progenitor cells (CPCs) are a crucial source of cells in cardiac development and regeneration. However, reported CPCs are heterogeneous, and no gene has been identified to transiently mark undifferentiated CPCs throughout heart development. Here we show that Spalt-like gene 1 (Sall1), a zing-finger transcription factor, is expressed in undifferentiated CPCs giving rise to both left and right ventricles. Sall1 was transiently expressed in precardiac mesoderm contributing to the first heart field (left ventricle precursors) but not in the field itself. Similarly, Sall1 expression was maintained in the second heart field (outflow tract/right ventricle precursors) but not in cardiac cells. In vitro, high levels of Sall1 at mesodermal stages enhanced cardiomyogenesis, whereas its continued expression suppressed cardiac differentiation. Our study demonstrates that Sall1 marks CPCs in an undifferentiated state and regulates cardiac differentiation. These findings provide fundamental insights into CPC maintenance, which can be instrumental for CPC-based regenerative medicine.
KW - Cardiac development
KW - Cardiac progenitor
KW - Cardiac transcription factors
KW - ES/iPS cells
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U2 - 10.1016/j.yjmcc.2016.02.008
DO - 10.1016/j.yjmcc.2016.02.008
M3 - Article
C2 - 26876450
AN - SCOPUS:84966938172
SN - 0022-2828
VL - 92
SP - 158
EP - 162
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
ER -