Abstract
Retinal detachment secondary to proliferative vitreoretinopathy (PVR) is often precipitated by traction on the retina from preretinal membranes and traction on the vitreous base. Contraction and proliferation of retinal pigment epithelial (RPE) cells appear to play an important role in the pathogenesis of PVR. Contraction of collagen gels by human RPE cells was evaluated as a model of tractional forces on the vitreous gel. The area of collagen gels with admixed RPE cells in 15-mm wells was measured daily. The collagen gels contracted to < 50% of original area in 89% of wells. Colchicine (0.01-1 μM) inhibited RPE-induced collagen gel contraction, whereas 1 ng/ml of transforming growth factor-beta enhanced it. Potantial stimulators or inhibitors of RPE-mediated gel contraction can be tested using this model.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1079-1086 |
| Number of pages | 8 |
| Journal | Investigative Ophthalmology and Visual Science |
| Volume | 31 |
| Issue number | 6 |
| State | Published - 1990 |
Keywords
- colchicine
- collagen gel
- proliferative vitreoretinopathy
- retinal pigment epithelium
- transforming growth factor-beta
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience