Role of vascular endothelial growth factor in neuronal DNA damage and repair in rat brain following a transient cerebral ischemia

Zeng Jin Yang, Wei Li Bao, Mei Hong Qiu, Ling Mei Zhang, Shi Duo Lu, Ya Lin Huang, Feng Yan Sun

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

The antisense knockdown technique and confocal laser scanning microscopic analysis were used to elucidate vascular endothelial growth factor (VEGF) induction and its effect on DNA damage and repair in rat brain following a transient middle cerebral artery occlusion. Immunohistochemical study and in situ hybridization showed that the expression of VEGF and its mRNA was enhanced in the ischemic core and penumbra of ischemic brain. Western blot analysis further illustrated that VEGF induction was time-dependently changed in these areas. Double-staining analysis indicated that VEGF-positive staining existed in the neuron, but not in the glia, and it colocalized with excision repair cross-complementing group 6 (ERCC6) mRNA, a DNA repair factor. VEGF antisense oligodeoxynucleotide infusion reduced VEGF induction and resulted in an enlargement of infarct volume of the brain caused by ischemia. Moreover, it also increased the number of DNA damaged cells and lessened the induction of ERCC6 mRNA in ischemic brains. These results suggest that the induction of endogenous VEGF in ischemic neurons plays a neuroprotective role probably associated with the expression of ERCC6 mRNA.

Original languageEnglish (US)
Pages (from-to)140-149
Number of pages10
JournalJournal of neuroscience research
Volume70
Issue number2
DOIs
StatePublished - Oct 15 2002
Externally publishedYes

Keywords

  • Brain
  • DNA repair
  • Ischemia
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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