Abstract
In this article, Johnson and colleagues co-cultured human embryonic stem cell-derived retinal ganglion cells (RGCs) on the surface of adult organotypic retinal explants. The authors showed that RGCs spontaneously clustered and extended neurites that were excluded from the retinal parenchyma, but that internal limiting membrane (ILM) digestion permitted robust neurite engraftment with little toxicity, thereby identifying the ILM as a barrier to transplanted RGC retinal integration.
Original language | English (US) |
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Pages (from-to) | 149-167 |
Number of pages | 19 |
Journal | Stem Cell Reports |
Volume | 16 |
Issue number | 1 |
DOIs | |
State | Published - Jan 12 2021 |
Keywords
- cellular interactions
- dendrite
- engraftment
- glaucoma
- migration
- optic nerve regeneration
- optic neuropathy
- regenerative medicine
- retinal ganglion cell
- transplantation
ASJC Scopus subject areas
- Biochemistry
- Genetics
- Developmental Biology
- Cell Biology