Role of protein kinase N2 (PKN2) in cigarette smoke-mediated oncogenic transformation of oral cells

Pavithra Rajagopalan, Vishalakshi Nanjappa, Krishna Patel, Ankit P. Jain, Kiran K. Mangalaparthi, Arun H. Patil, Bipin Nair, Premendu P. Mathur, T. S. Keshava Prasad, Joseph A. Califano, David Sidransky, Harsha Gowda, Aditi Chatterjee

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Smoking is the leading cause of preventable death worldwide. Though cigarette smoke is an established cause of head and neck cancer (including oral cancer), molecular alterations associated with chronic cigarette smoke exposure are poorly studied. To understand the signaling alterations induced by chronic exposure to cigarette smoke, we developed a cell line model by exposing normal oral keratinocytes to cigarette smoke for a period of 12 months. Chronic exposure to cigarette smoke resulted in increased cellular proliferation and invasive ability of oral keratinocytes. Proteomic and phosphoproteomic analyses showed dysregulation of several proteins involved in cellular movement and cytoskeletal reorganization in smoke exposed cells. We observed overexpression and hyperphosphorylation of protein kinase N2 (PKN2) in smoke exposed cells as well as in a panel of head and neck cancer cell lines established from smokers. Silencing of PKN2 resulted in decreased colony formation, invasion and migration in both smoke exposed cells and head and neck cancer cell lines. Our results indicate that PKN2 plays an important role in oncogenic transformation of oral keratinocytes in response to cigarette smoke. The current study provides evidence that PKN2 can act as a potential therapeutic target in head and neck squamous cell carcinoma, especially in patients with a history of smoking.

Original languageEnglish (US)
Pages (from-to)709-721
Number of pages13
JournalJournal of Cell Communication and Signaling
Issue number4
StatePublished - Dec 1 2018


  • Carcinogenesis
  • Cell adhesion
  • High-throughput
  • Orbitrap fusion
  • Smoking

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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