Role of antigen-specific suppressor T cells in the formation of IgE-suppressive factor

P. Jardieu, K. Ishizaka

Research output: Contribution to journalArticlepeer-review

Abstract

Three intravenous injections of ovalbumin (OA) into S6D2F1 mice induced antigen-specific suppressor T cells, and incubation of their spleen cells with OA resulted in the formation of IgE-suppressive factor. However, if the lyt 2+ cells or I-J+ cells in the spleen were depleted prior to incubation with OA, the remaining spleen cells formed IgE-potentiating factor. Analysis of cellular mechanims (or the formation of IgE-binding factors by antigenic stimulation indicated that antigen-primed Lyt 1+ T cells form 'inducers' of IgE-binding factor and glycosylation-enhancing factor (GEF), and that these factors in turn, induce unprimed T cells to form IgE-potentiating factor. As Lyt 1+ cell-depleted fraction of OA-treated mice formed neither inducer nor IgE-binding factor, but released glycosylation inhibiting factor (GIF), which prevented the assembly of N-linked oligosaccharide to IgE-binding factors during their biosynthesis. These data indicate that the major source of IgE-suppressive factor are Lyt+ T cells, and that Ly 2+ I-J+ cells provide GIF, which overrides the effects of GEF from Lyt 1+ cells, resulting in the selective formation of IgE-suppressive factor. The GIF released from Ly 2+ T cells had a molecular weight of approximately 30.000, bound to a monoclonal anti-lipomodulin and to anti-I-J(b) alloantibodies, but failed to bind to anti-I-J8 antibodies. The results suggest that suppressor T cell-derived lipomodulin contains I-J determinants.

Original languageEnglish (US)
Pages (from-to)no. 3226
JournalFederation Proceedings
Volume43
Issue number7
StatePublished - Jan 1 1984

ASJC Scopus subject areas

  • General Medicine

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