Role of adenovirus type 5 early region 3 in the pathogenesis of ocular disease and cell culture infection

M. D. Trousdale, R. Nobrega, D. Stevenson, T. Nakamura, P. M. Dos Santos, L. Labree, P. J. McDonnell

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Experimental animal virus replication models make it possible to study the role of vital gene products in adenovirus (Ad)-induced ocular disease. This study tested the hypothesis that the early region 3 (E3) of the human Ad genome plays an important role in the pathogenesis of Ad-induced ocular disease. Both Ad5 wt300, a genetically defined E3+ parent, and Ad5 dl327, a deletion mutant without E3 (E3-), replicated in ocular-derived cell cultures. Ad5 E3+ and Ad8 replicated more efficiently than did Ad5 E3- in cornea and conjunctival cell cultures. Lacrimal gland-derived cell cultures supported human Ad8 replication significantly more efficiently than did either AdS E3+ or Ad5 E3-. After intrastromal and topical inoculation of rabbits with either Ad wt300 or Ad dl327, a specific immune response was elicited that coincided with the appearance of subepithelial opacities that mimicked human disease both clinically and histologically. The clinical features (i.e., conjunctivitis, iritis, and corneal edema) were not significantly different for Ad5 E3+- and Ad5 E3- -induced ocular infection. Ad5 E3+- and Ad5 E3- -inoculated eyes shed virus for up to 7 and 5 days, respectively, and occasionally established persistent and/or latent infections in corneal, conjunctival, and, infrequently, lacrimal gland cells. Both Ad5 E3+ and Ad5 E3 spread from virus-inoculated animals to the cornea and conjunctiva of normal animals. Under current experimental conditions, expression of the E3 gene does not appear to affect the degree of virulence in ocular disease induced by Ad5 in the rabbit eye model. Deletion of the E3 gene from Ad5 does not make the model more like human disease.

Original languageEnglish (US)
Pages (from-to)280-289
Number of pages10
Issue number3
StatePublished - 1995
Externally publishedYes


  • Adenovirus
  • Animal model
  • Early region 3
  • Epidemic keratoconjunctivitis

ASJC Scopus subject areas

  • Ophthalmology


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