RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C. elegans glycogene database

Sayaka Akiyoshi; Kazuko H. Nomura; Katsufumi Dejima; Daisuke Murata; Ayako Matsuda; Nanako Kanaki; Tetsuro Takaki; Hiroyuki Mihara; Takayuki Nagaishi; Shuhei Furukawa; Keiko Gengyo Ando; Sawako Yoshina; Shohei Mitani; Akira Togayachi; Yoshinori Suzuki; Toshihide Shikanai; Hisashi Narimatsu; Kazuya Nomura

doi:10.1093/glycob/cwu080

RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C. elegans glycogene database

Sayaka Akiyoshi, Kazuko H. Nomura, Katsufumi Dejima, Daisuke Murata, Ayako Matsuda, Nanako Kanaki, Tetsuro Takaki, Hiroyuki Mihara, Takayuki Nagaishi, Shuhei Furukawa, Keiko Gengyo Ando, Sawako Yoshina, Shohei Mitani, Akira Togayachi, Yoshinori Suzuki, Toshihide Shikanai, Hisashi Narimatsu, Kazuya Nomura

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, we selected 181 nematode glycogenes that are orthologous to human glycogenes and examined their RNAi phenotypes. The results are deposited in the Caenorhabditis elegans Glycogene Database (CGGDB) at AIST, Tsukuba, Japan. The most prominent RNAi phenotypes observed are disruptions of cell cycle progression in germline mitosis/meiosis and in early embryonic cell mitosis. Along with the previously reported roles of chondroitin proteoglycans, glycosphingolipids and GPI-anchored proteins in cell cycle progression, we show for the first time that the inhibition of the functions of N-glycan synthesis genes (cytoplasmic alg genes) resulted in abnormal germline formation, ER stress and small body size phenotypes. The results provide additional information on the roles of glycoconjugates in the cell cycle progression mechanisms of germline and embryonic cells.

Original language	English (US)
Pages (from-to)	8-20
Number of pages	13
Journal	Glycobiology
Volume	25
Issue number	1
DOIs	https://doi.org/10.1093/glycob/cwu080
State	Published - Jan 1 2015
Externally published	Yes

Keywords

RNAi
alg genes
germline cell division
glycogene
oligosaccharyltransferase

ASJC Scopus subject areas

Biochemistry

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10.1093/glycob/cwu080

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Akiyoshi, S., Nomura, K. H., Dejima, K., Murata, D., Matsuda, A., Kanaki, N., Takaki, T., Mihara, H., Nagaishi, T., Furukawa, S., Ando, K. G., Yoshina, S., Mitani, S., Togayachi, A., Suzuki, Y., Shikanai, T., Narimatsu, H., & Nomura, K. (2015). RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C. elegans glycogene database. Glycobiology, 25(1), 8-20. https://doi.org/10.1093/glycob/cwu080

Akiyoshi, S, Nomura, KH, Dejima, K, Murata, D, Matsuda, A, Kanaki, N, Takaki, T, Mihara, H, Nagaishi, T, Furukawa, S, Ando, KG, Yoshina, S, Mitani, S, Togayachi, A, Suzuki, Y, Shikanai, T, Narimatsu, H & Nomura, K 2015, 'RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C. elegans glycogene database', Glycobiology, vol. 25, no. 1, pp. 8-20. https://doi.org/10.1093/glycob/cwu080

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abstract = "In this study, we selected 181 nematode glycogenes that are orthologous to human glycogenes and examined their RNAi phenotypes. The results are deposited in the Caenorhabditis elegans Glycogene Database (CGGDB) at AIST, Tsukuba, Japan. The most prominent RNAi phenotypes observed are disruptions of cell cycle progression in germline mitosis/meiosis and in early embryonic cell mitosis. Along with the previously reported roles of chondroitin proteoglycans, glycosphingolipids and GPI-anchored proteins in cell cycle progression, we show for the first time that the inhibition of the functions of N-glycan synthesis genes (cytoplasmic alg genes) resulted in abnormal germline formation, ER stress and small body size phenotypes. The results provide additional information on the roles of glycoconjugates in the cell cycle progression mechanisms of germline and embryonic cells.",

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AU - Murata, Daisuke

AU - Matsuda, Ayako

AU - Kanaki, Nanako

AU - Takaki, Tetsuro

AU - Mihara, Hiroyuki

AU - Nagaishi, Takayuki

AU - Furukawa, Shuhei

AU - Ando, Keiko Gengyo

AU - Yoshina, Sawako

AU - Mitani, Shohei

AU - Togayachi, Akira

AU - Suzuki, Yoshinori

AU - Shikanai, Toshihide

AU - Narimatsu, Hisashi

AU - Nomura, Kazuya

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RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C. elegans glycogene database

Abstract

Keywords

ASJC Scopus subject areas

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