RNA purification - Precipitation methods

Sarah E. Walker, Jon Lorsch

Research output: Chapter in Book/Report/Conference proceedingChapter

29 Scopus citations

Abstract

When working with RNA, the need often arises to concentrate a sample or purify it from various salts, nucleotides, and proteins. RNA precipitation is an easy and cost-effective method for the concentration of RNA, leaving a pellet that can be resuspended in the buffer of choice.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationRNA
PublisherAcademic Press Inc.
Pages337-343
Number of pages7
ISBN (Print)9780124200371
DOIs
StatePublished - 2013

Publication series

NameMethods in Enzymology
Volume530
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Ammonium acetate
  • Ethanol precipitation
  • Lithium chloride precipitation
  • Precipitation methods
  • RNA purification
  • Sodium acetate

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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