TY - JOUR
T1 - RNA editing involves indiscriminate U changes throughout precisely defined editing domains
AU - Decker, Carolyn J.
AU - Sollner-Webb, Barbara
N1 - Funding Information:
This work was supported by National Institutes of Health grant GM-34231; C. DB salary was partly defrayed by funds from the MacArthur Foundation.
PY - 1990/6/15
Y1 - 1990/6/15
N2 - RNA editing is the specific posttranscriptional insertion/deletion of U residues within trypanosomatid mitochondrial transcripts. We used a novel cDNA cloning scheme and analyzed partially edited COIII and CYb RNAs. Our major unanticipated findings are: First, editing appears strikingly indiscriminate within editing domains, inserting and deleting variable numbers of U residues at all sites, both those requiring editing and those that do not. Second, this incomplete editing occurs in precise domains, not in adjacent regions unedited in mature mRNA. Third, editing appears to commence prior to polyadenylation. We propose that within a region being edited, there are cycles of indiscriminate cleavage, U addition or deletion, and religation; sites that become correctly edited would be protected from further modification by duplexing with short RNAs complementary to the edited sequence.
AB - RNA editing is the specific posttranscriptional insertion/deletion of U residues within trypanosomatid mitochondrial transcripts. We used a novel cDNA cloning scheme and analyzed partially edited COIII and CYb RNAs. Our major unanticipated findings are: First, editing appears strikingly indiscriminate within editing domains, inserting and deleting variable numbers of U residues at all sites, both those requiring editing and those that do not. Second, this incomplete editing occurs in precise domains, not in adjacent regions unedited in mature mRNA. Third, editing appears to commence prior to polyadenylation. We propose that within a region being edited, there are cycles of indiscriminate cleavage, U addition or deletion, and religation; sites that become correctly edited would be protected from further modification by duplexing with short RNAs complementary to the edited sequence.
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U2 - 10.1016/0092-8674(90)90065-M
DO - 10.1016/0092-8674(90)90065-M
M3 - Article
C2 - 1693545
AN - SCOPUS:0025294781
SN - 0092-8674
VL - 61
SP - 1001
EP - 1011
JO - Cell
JF - Cell
IS - 6
ER -