Risk of Leakage and Tissue Dissemination With Various Contained Tissue Extraction (CTE) Techniques: An inVitro Pilot Study

Sarah L. Cohen, James A. Greenberg, Karen C. Wang, Serene S. Srouji, Antonio R. Gargiulo, Charles N. Pozner, Nicholas Hoover, Jon I. Einarsson

Research output: Contribution to journalArticlepeer-review

49 Scopus citations


Study Objective: To evaluate risk of leakage and tissue dissemination associated with various contained tissue extraction (CTE) techniques. Design: Invitro study (Canadian Task Force classification: II-1). Setting: Academic hospital simulation laboratory. Intervention: Beef tongue specimens weighing 400 to 500g were stained using 5mL indigo carmine dye and morcellated under laparoscopic guidance within a plastic box trainer. CTE was performed via 3 different techniques: a stitch-sealed rip-stop nylon bag and multi-port approach; a one-piece clear plastic 50×50-cm isolation bag and multi-port approach; or a 1-piece clear plastic 50×50-cm isolation bag and single-site approach. Four trials of each CTE method were performed and compared with an open morcellation control. All bags were insufflated to within 10 to 25mmHg pressure with a standard CO2 insufflator. Visual evidence of spilled tissue or dye was recorded, and fluid washings of the box trainer were sent for cytologic analysis. Measurements and Main Results: Blue dye spill was noted in only 1 of 12 CTE trials. Spillage was visualized from a seam in 1 of the 4 stitch-sealed rip-stop nylon bags before morcellation of the specimen. The only trial in which gross tissue chips were visualized in the box trainer after morcellation was the open morcellation control. However, cytologic examination revealed muscle cells in the open morcellation washings and in the washings from the trial with dye spill. Muscle cells were not observed at cytologly in any of the other samples. Conclusion: CTE did not result in any leakage or tissue dissemination with use of the single-site or multi-port approach when using a 1-piece clear plastic 50×50-cm isolation bag. Further studies are needed to corroborate these findings in an invivo context and to evaluate use of alternate bag options for specimen containment.

Original languageEnglish (US)
Pages (from-to)935-939
Number of pages5
JournalJournal of Minimally Invasive Gynecology
Issue number5
StatePublished - Sep 1 2014
Externally publishedYes


  • Hysterectomy
  • Laparoscopy
  • Morcellation
  • Myomectomy
  • Tissue extraction

ASJC Scopus subject areas

  • Obstetrics and Gynecology


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