Avian myeloblastosis virus and Maloney murlne leukemia virus RNase H- reverse transcriptases pause when they encounter a 2′-5′ linkage or a 2′-thiophosphate In their template RNAs, but eventually read through these backbone modifications. Both reverse transcriptases pause after the 2′-5′ linkage but before the 2′-thlophosphate. These results suggest that in the absence of precise information concerning the behavior of a given reverse transcriptase with respect to a particular lesion or modification, caution should be exercised in the interpretation of primer extension data that is being used to determine the existence of, or map the position of, a crosslink, site of chemical modification or non-standard linkage in an RNA template.
|Original language||English (US)|
|Number of pages||4|
|Journal||Nucleic acids research|
|State||Published - Aug 11 1995|
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