Reverse transcriptase dideoxy sequencing of RNA

Sarah E. Walker, Jon Lorsch

Research output: Chapter in Book/Report/Conference proceedingChapter


During the course of studying the structure and function of RNA molecules and the proteins that they may encode, as well as studies of the expression of a given gene, there often arises a need to learn the nucleotide sequence of an RNA molecule. While the sequence of a particular RNA is ordinarily encompassed within a corresponding DNA sequence, RNA sequencing lends the advantage of giving the transcription initiation and termination sequences, and the sites where modifications from the original DNA sequence occur.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationRNA
PublisherAcademic Press Inc.
Number of pages13
ISBN (Print)9780124200371
StatePublished - 2013

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988


  • Anneal primer
  • Label primer
  • Primer extension and chain termination
  • Reverse transcriptase dideoxy sequencing

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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