Resuscitation of dormant Mycobacterium tuberculosis by phospholipids or specific peptides

Ying Zhang; Yongping Yang; Amina Woods; Robert J. Cotter; Zhonghe Sun

doi:10.1006/bbrc.2001.4993

Resuscitation of dormant Mycobacterium tuberculosis by phospholipids or specific peptides

Ying Zhang, Yongping Yang, Amina Woods, Robert J. Cotter, Zhonghe Sun

School of Medicine

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

The presence of dormant tubercle bacilli presents a major problem for tuberculosis treatment. The culture supernatant of Mycobacterium tuberculosis was previously shown to resuscitate dormant bacilli in vitro. Here we report identification of active components as phospholipids and a tuberculosis protein Rv1174c. Remarkably, dormant bacilli from a one year old culture which failed to form any colonies could be resuscitated with peptides derived from Rv1174c and formed 10^5-7 colonies/ml. This finding represents the first unambiguous demonstration of resuscitation of dormant tubercle bacilli in vitro and may have implication for the study of mycobacterial dormancy and the design of novel strategies for improved treatment of tuberculosis.

Original language	English (US)
Pages (from-to)	542-547
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	284
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.2001.4993
State	Published - 2001

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Resuscitation of dormant Mycobacterium tuberculosis by phospholipids or specific peptides",

abstract = "The presence of dormant tubercle bacilli presents a major problem for tuberculosis treatment. The culture supernatant of Mycobacterium tuberculosis was previously shown to resuscitate dormant bacilli in vitro. Here we report identification of active components as phospholipids and a tuberculosis protein Rv1174c. Remarkably, dormant bacilli from a one year old culture which failed to form any colonies could be resuscitated with peptides derived from Rv1174c and formed 105-7 colonies/ml. This finding represents the first unambiguous demonstration of resuscitation of dormant tubercle bacilli in vitro and may have implication for the study of mycobacterial dormancy and the design of novel strategies for improved treatment of tuberculosis.",

author = "Ying Zhang and Yongping Yang and Amina Woods and Cotter, {Robert J.} and Zhonghe Sun",

note = "Funding Information: We thank Qiujia Shao for excellent technical assistance and William Jacobs for critical reading of the manuscript. This work was supported by research grants from the National Institutes of Health (AI40584 and AI44063) and the Potts Memorial Foundation.",

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AU - Zhang, Ying

AU - Yang, Yongping

AU - Woods, Amina

AU - Cotter, Robert J.

AU - Sun, Zhonghe

N1 - Funding Information: We thank Qiujia Shao for excellent technical assistance and William Jacobs for critical reading of the manuscript. This work was supported by research grants from the National Institutes of Health (AI40584 and AI44063) and the Potts Memorial Foundation.

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AB - The presence of dormant tubercle bacilli presents a major problem for tuberculosis treatment. The culture supernatant of Mycobacterium tuberculosis was previously shown to resuscitate dormant bacilli in vitro. Here we report identification of active components as phospholipids and a tuberculosis protein Rv1174c. Remarkably, dormant bacilli from a one year old culture which failed to form any colonies could be resuscitated with peptides derived from Rv1174c and formed 105-7 colonies/ml. This finding represents the first unambiguous demonstration of resuscitation of dormant tubercle bacilli in vitro and may have implication for the study of mycobacterial dormancy and the design of novel strategies for improved treatment of tuberculosis.

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Resuscitation of dormant Mycobacterium tuberculosis by phospholipids or specific peptides

Abstract

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