Response of zonal chondrocytes to extracellular matrix-hydrogels

Nathaniel S. Hwang; Shyni Varghese; H. Janice Lee; Parnduangjai Theprungsirikul; Adam Canver; Blanka Sharma; Jennifer Elisseeff

doi:10.1016/j.febslet.2007.07.049

Response of zonal chondrocytes to extracellular matrix-hydrogels

Nathaniel S. Hwang, Shyni Varghese, H. Janice Lee, Parnduangjai Theprungsirikul, Adam Canver, Blanka Sharma, Jennifer Elisseeff

School of Medicine

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

We investigated the biological response of chondrocytes isolated from different zones of articular cartilage and their cellular behaviors in poly (ethylene glycol)-based (PEG) hydrogels containing exogenous type I collagen, hyaluronic acid (HA), or chondroitin sulfate (CS). The cellular morphology was strongly dependent on the extracellular matrix component of hydrogels. Additionally, the exogenous extracellular microenvironment affected matrix production and cartilage specific gene expression of chondrocytes from different zones. CS-based hydrogels showed the strongest response in terms of gene expression and matrix accumulation for both superficial and deep zone chondrocytes, but HA and type I collagen-based hydrogels demonstrated zonal-dependent cellular responses.

Original language	English (US)
Pages (from-to)	4172-4178
Number of pages	7
Journal	FEBS Letters
Volume	581
Issue number	22
DOIs	https://doi.org/10.1016/j.febslet.2007.07.049
State	Published - Sep 4 2007

Keywords

Articular cartilage
Chondrocyte
Extracellular matrices
Gene expression
Hydrogel
Zonal organization

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2007.07.049

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title = "Response of zonal chondrocytes to extracellular matrix-hydrogels",

abstract = "We investigated the biological response of chondrocytes isolated from different zones of articular cartilage and their cellular behaviors in poly (ethylene glycol)-based (PEG) hydrogels containing exogenous type I collagen, hyaluronic acid (HA), or chondroitin sulfate (CS). The cellular morphology was strongly dependent on the extracellular matrix component of hydrogels. Additionally, the exogenous extracellular microenvironment affected matrix production and cartilage specific gene expression of chondrocytes from different zones. CS-based hydrogels showed the strongest response in terms of gene expression and matrix accumulation for both superficial and deep zone chondrocytes, but HA and type I collagen-based hydrogels demonstrated zonal-dependent cellular responses.",

keywords = "Articular cartilage, Chondrocyte, Extracellular matrices, Gene expression, Hydrogel, Zonal organization",

author = "Hwang, {Nathaniel S.} and Shyni Varghese and Lee, {H. Janice} and Parnduangjai Theprungsirikul and Adam Canver and Blanka Sharma and Jennifer Elisseeff",

note = "Funding Information: This study was supported by the National Institutes of Health (R01 EB05517) and the Arthritis Foundation. The authors are grateful to Dr. Zijun Zhang for critical review and technical assistance. ",

year = "2007",

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doi = "10.1016/j.febslet.2007.07.049",

language = "English (US)",

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T1 - Response of zonal chondrocytes to extracellular matrix-hydrogels

AU - Hwang, Nathaniel S.

AU - Varghese, Shyni

AU - Lee, H. Janice

AU - Theprungsirikul, Parnduangjai

AU - Canver, Adam

AU - Sharma, Blanka

AU - Elisseeff, Jennifer

N1 - Funding Information: This study was supported by the National Institutes of Health (R01 EB05517) and the Arthritis Foundation. The authors are grateful to Dr. Zijun Zhang for critical review and technical assistance.

PY - 2007/9/4

Y1 - 2007/9/4

N2 - We investigated the biological response of chondrocytes isolated from different zones of articular cartilage and their cellular behaviors in poly (ethylene glycol)-based (PEG) hydrogels containing exogenous type I collagen, hyaluronic acid (HA), or chondroitin sulfate (CS). The cellular morphology was strongly dependent on the extracellular matrix component of hydrogels. Additionally, the exogenous extracellular microenvironment affected matrix production and cartilage specific gene expression of chondrocytes from different zones. CS-based hydrogels showed the strongest response in terms of gene expression and matrix accumulation for both superficial and deep zone chondrocytes, but HA and type I collagen-based hydrogels demonstrated zonal-dependent cellular responses.

AB - We investigated the biological response of chondrocytes isolated from different zones of articular cartilage and their cellular behaviors in poly (ethylene glycol)-based (PEG) hydrogels containing exogenous type I collagen, hyaluronic acid (HA), or chondroitin sulfate (CS). The cellular morphology was strongly dependent on the extracellular matrix component of hydrogels. Additionally, the exogenous extracellular microenvironment affected matrix production and cartilage specific gene expression of chondrocytes from different zones. CS-based hydrogels showed the strongest response in terms of gene expression and matrix accumulation for both superficial and deep zone chondrocytes, but HA and type I collagen-based hydrogels demonstrated zonal-dependent cellular responses.

KW - Articular cartilage

KW - Chondrocyte

KW - Extracellular matrices

KW - Gene expression

KW - Hydrogel

KW - Zonal organization

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SN - 0014-5793

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JO - FEBS Letters

JF - FEBS Letters

IS - 22

ER -

Response of zonal chondrocytes to extracellular matrix-hydrogels

Abstract

Keywords

ASJC Scopus subject areas

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