Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site

Craig Montell; Eric F. Fisher; Marvin H. Caruthers; Arnold J. Berk

doi:10.1038/295380a0

Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site

Craig Montell, Eric F. Fisher, Marvin H. Caruthers, Arnold J. Berk

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134 Scopus citations

Abstract

Early region IA of human adenoviruses encodes a function required for normal induction of early viral genes and virus-induced cell transformation. The region is expressed at early times as two overlapping spliced mRNAs, 12S and 13S, which encode closely related proteins. To distinguish between the functions of these proteins, a single T → G transversion was constructed which prevents splicing of the 12S mRNA. This transversion, in the second base of the 12S mRNA intron, does not alter the protein encoded by the 13S mRNA due to degeneracy in the genetic code. Studies with this mutant demonstrated that only the 13S mRNA encodes the regulatory protein required for normal early gene expression.

Original language	English (US)
Pages (from-to)	380-384
Number of pages	5
Journal	Nature
Volume	295
Issue number	5848
DOIs	https://doi.org/10.1038/295380a0
State	Published - 1982
Externally published	Yes

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title = "Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site",

abstract = "Early region IA of human adenoviruses encodes a function required for normal induction of early viral genes and virus-induced cell transformation. The region is expressed at early times as two overlapping spliced mRNAs, 12S and 13S, which encode closely related proteins. To distinguish between the functions of these proteins, a single T → G transversion was constructed which prevents splicing of the 12S mRNA. This transversion, in the second base of the 12S mRNA intron, does not alter the protein encoded by the 13S mRNA due to degeneracy in the genetic code. Studies with this mutant demonstrated that only the 13S mRNA encodes the regulatory protein required for normal early gene expression.",

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AU - Fisher, Eric F.

AU - Caruthers, Marvin H.

AU - Berk, Arnold J.

PY - 1982

Y1 - 1982

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AB - Early region IA of human adenoviruses encodes a function required for normal induction of early viral genes and virus-induced cell transformation. The region is expressed at early times as two overlapping spliced mRNAs, 12S and 13S, which encode closely related proteins. To distinguish between the functions of these proteins, a single T → G transversion was constructed which prevents splicing of the 12S mRNA. This transversion, in the second base of the 12S mRNA intron, does not alter the protein encoded by the 13S mRNA due to degeneracy in the genetic code. Studies with this mutant demonstrated that only the 13S mRNA encodes the regulatory protein required for normal early gene expression.

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Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site

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