Renal adaptation to phosphate load in the acutely thyroparathyroidectomized rat: Rapid alteration in brush border membrane phosphate transport

The sustained in vivo infusion of phosphate into thyroparathyroidectomized rats resulted, after 1 h, in a marked decrease in net phosphate reabsorption, even though the plasma concentration of phosphate continued to rise. This response to phosphate infusion was expressed at the level of the proximal tubule brush border membrane. Within 40 min of the initiation of the infusion the Na⁺-dependent phosphate uptake system in isolated membrane vesicles was decreased. Phosphate uptake in the absence of Na⁺, Na⁺-dependent D-glucose uptake, and ²²Na⁺ uptake were not affected. These findings demonstrate the locus of this parathyroid hormone-independent adaptation and indicate the rapidity with which the membrane transport system is regulated.