Release of DNA template restrictions in rabbit spermatozoa and rat liver nuclei

Barry R. Zirkin; Adele Boison; W. D.W. Heston; Donald S. Coffey

doi:10.1002/jez.1401970209

Release of DNA template restrictions in rabbit spermatozoa and rat liver nuclei

Barry R. Zirkin, Adele Boison, W. D.W. Heston, Donald S. Coffey

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The effects of disulfide reduction on DNA template activity of rabbit sperm and rat liver nuclei are compared. After treatment of rabbit spermatozoa with 5 mM dithiothreitol and 1% Triton X‐100 for 90 minutes at room temperature, sperm nuclei decondense and nuclear template restrictions for DNA synthesis are released. By contrast, similar thiol treatment of isolated rat liver nuclei fails to release DNA template restrictions. These results suggest a significant difference in the control of template activity in mammalian sperm and somatic cell nuclei.

Original language	English (US)
Pages (from-to)	283-288
Number of pages	6
Journal	Journal of Experimental Zoology
Volume	197
Issue number	2
DOIs	https://doi.org/10.1002/jez.1401970209
State	Published - Aug 1976
Externally published	Yes

ASJC Scopus subject areas

Animal Science and Zoology

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title = "Release of DNA template restrictions in rabbit spermatozoa and rat liver nuclei",

abstract = "The effects of disulfide reduction on DNA template activity of rabbit sperm and rat liver nuclei are compared. After treatment of rabbit spermatozoa with 5 mM dithiothreitol and 1% Triton X‐100 for 90 minutes at room temperature, sperm nuclei decondense and nuclear template restrictions for DNA synthesis are released. By contrast, similar thiol treatment of isolated rat liver nuclei fails to release DNA template restrictions. These results suggest a significant difference in the control of template activity in mammalian sperm and somatic cell nuclei.",

author = "Zirkin, {Barry R.} and Adele Boison and Heston, {W. D.W.} and Coffey, {Donald S.}",

year = "1976",

month = aug,

doi = "10.1002/jez.1401970209",

language = "English (US)",

volume = "197",

pages = "283--288",

journal = "Journal of Experimental Zoology",

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T1 - Release of DNA template restrictions in rabbit spermatozoa and rat liver nuclei

AU - Zirkin, Barry R.

AU - Boison, Adele

AU - Heston, W. D.W.

AU - Coffey, Donald S.

PY - 1976/8

Y1 - 1976/8

N2 - The effects of disulfide reduction on DNA template activity of rabbit sperm and rat liver nuclei are compared. After treatment of rabbit spermatozoa with 5 mM dithiothreitol and 1% Triton X‐100 for 90 minutes at room temperature, sperm nuclei decondense and nuclear template restrictions for DNA synthesis are released. By contrast, similar thiol treatment of isolated rat liver nuclei fails to release DNA template restrictions. These results suggest a significant difference in the control of template activity in mammalian sperm and somatic cell nuclei.

AB - The effects of disulfide reduction on DNA template activity of rabbit sperm and rat liver nuclei are compared. After treatment of rabbit spermatozoa with 5 mM dithiothreitol and 1% Triton X‐100 for 90 minutes at room temperature, sperm nuclei decondense and nuclear template restrictions for DNA synthesis are released. By contrast, similar thiol treatment of isolated rat liver nuclei fails to release DNA template restrictions. These results suggest a significant difference in the control of template activity in mammalian sperm and somatic cell nuclei.

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EP - 288

JO - Journal of Experimental Zoology

JF - Journal of Experimental Zoology

IS - 2

ER -

Release of DNA template restrictions in rabbit spermatozoa and rat liver nuclei

Abstract

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