Regulation of Neurotransmitter Aspartate Metabolism by Glial Glutamine Synthetase

Jeffrey D. Rothstein; Boris Tabakoff

doi:10.1111/j.1471-4159.1986.tb08514.x

Regulation of Neurotransmitter Aspartate Metabolism by Glial Glutamine Synthetase

Jeffrey D. Rothstein, Boris Tabakoff

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Aspartate levels and release from rat striatal slices following the inhibition of glutamine synthetase (GS) by methionine sulfoximine (MSO) were studied. Striatal levels of aspartate and glutamine were decreased over time in a manner that correlated with GS inhibition. Ca²⁺‐dependent, K⁺‐stimulated aspartate release was diminished in striatal tissue slices from animals pretreated with MSO. The decreased release of aspartate correlated over time with the inhibition of GS. The addition of glutamine to the perfusion medium completely reversed the effects of MSO on calcium‐dependent aspartate release. It is suggested that glutamine is a major precursor for transmitter aspartate.

Original language	English (US)
Pages (from-to)	1923-1928
Number of pages	6
Journal	Journal of Neurochemistry
Volume	46
Issue number	6
DOIs	https://doi.org/10.1111/j.1471-4159.1986.tb08514.x
State	Published - Jun 1986
Externally published	Yes

Keywords

Aspartate
Glial cells
Glutamine
Glutamine synthetase
Methionine sulfoximine

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

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10.1111/j.1471-4159.1986.tb08514.x

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title = "Regulation of Neurotransmitter Aspartate Metabolism by Glial Glutamine Synthetase",

abstract = "Aspartate levels and release from rat striatal slices following the inhibition of glutamine synthetase (GS) by methionine sulfoximine (MSO) were studied. Striatal levels of aspartate and glutamine were decreased over time in a manner that correlated with GS inhibition. Ca2+‐dependent, K+‐stimulated aspartate release was diminished in striatal tissue slices from animals pretreated with MSO. The decreased release of aspartate correlated over time with the inhibition of GS. The addition of glutamine to the perfusion medium completely reversed the effects of MSO on calcium‐dependent aspartate release. It is suggested that glutamine is a major precursor for transmitter aspartate.",

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AU - Rothstein, Jeffrey D.

AU - Tabakoff, Boris

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AB - Aspartate levels and release from rat striatal slices following the inhibition of glutamine synthetase (GS) by methionine sulfoximine (MSO) were studied. Striatal levels of aspartate and glutamine were decreased over time in a manner that correlated with GS inhibition. Ca2+‐dependent, K+‐stimulated aspartate release was diminished in striatal tissue slices from animals pretreated with MSO. The decreased release of aspartate correlated over time with the inhibition of GS. The addition of glutamine to the perfusion medium completely reversed the effects of MSO on calcium‐dependent aspartate release. It is suggested that glutamine is a major precursor for transmitter aspartate.

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KW - Glial cells

KW - Glutamine

KW - Glutamine synthetase

KW - Methionine sulfoximine

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Regulation of Neurotransmitter Aspartate Metabolism by Glial Glutamine Synthetase

Abstract

Keywords

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