Regulation of early complement components C3 and C4 in the synovial

Timothy Collins, Jerry A. Winkelstein, Kathleen E. Sullivan

Research output: Contribution to journalArticlepeer-review

Abstract

To determine the cytokine inducibility of early complement component (C3 and C4) expression in the synovium, explant tissue was maintained in culture for 7 days. C3 and C4 production was measured by specific enzyme-linked immunosorbent assay, and RNA was evaluated by semiquantitative PCR. The effects of leukemia inhibitory factor (LIF), gamma interferon (IFN-γ), IFN-α, and estrogen on C3 and C4 expression were evaluated. C3 levels were unaffected by 7 days of LIF, IFN-γ, or IFN-α treatment. In contrast, C4 levels were significantly induced in synovial samples treated for 7 days with either IFN-γ or IFN-α. LIF had no effect on C4 levels in this system. Estrogen was found to down-modulate the induction of expression due to IFN-γ. These data provide evidence for cytokine regulation of C4 expression in the synovium and for estrogen modulation of those effects.

Original languageEnglish (US)
Pages (from-to)5-9
Number of pages5
JournalClinical and Diagnostic Laboratory Immunology
Volume3
Issue number1
DOIs
StatePublished - 1996

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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