TY - JOUR
T1 - Regulation of early complement components C3 and C4 in the synovial
AU - Collins, Timothy
AU - Winkelstein, Jerry A.
AU - Sullivan, Kathleen E.
PY - 1996
Y1 - 1996
N2 - To determine the cytokine inducibility of early complement component (C3 and C4) expression in the synovium, explant tissue was maintained in culture for 7 days. C3 and C4 production was measured by specific enzyme-linked immunosorbent assay, and RNA was evaluated by semiquantitative PCR. The effects of leukemia inhibitory factor (LIF), gamma interferon (IFN-γ), IFN-α, and estrogen on C3 and C4 expression were evaluated. C3 levels were unaffected by 7 days of LIF, IFN-γ, or IFN-α treatment. In contrast, C4 levels were significantly induced in synovial samples treated for 7 days with either IFN-γ or IFN-α. LIF had no effect on C4 levels in this system. Estrogen was found to down-modulate the induction of expression due to IFN-γ. These data provide evidence for cytokine regulation of C4 expression in the synovium and for estrogen modulation of those effects.
AB - To determine the cytokine inducibility of early complement component (C3 and C4) expression in the synovium, explant tissue was maintained in culture for 7 days. C3 and C4 production was measured by specific enzyme-linked immunosorbent assay, and RNA was evaluated by semiquantitative PCR. The effects of leukemia inhibitory factor (LIF), gamma interferon (IFN-γ), IFN-α, and estrogen on C3 and C4 expression were evaluated. C3 levels were unaffected by 7 days of LIF, IFN-γ, or IFN-α treatment. In contrast, C4 levels were significantly induced in synovial samples treated for 7 days with either IFN-γ or IFN-α. LIF had no effect on C4 levels in this system. Estrogen was found to down-modulate the induction of expression due to IFN-γ. These data provide evidence for cytokine regulation of C4 expression in the synovium and for estrogen modulation of those effects.
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U2 - 10.1128/cdli.3.1.5-9.1996
DO - 10.1128/cdli.3.1.5-9.1996
M3 - Article
C2 - 8770496
AN - SCOPUS:0030065684
SN - 1071-412X
VL - 3
SP - 5
EP - 9
JO - Clinical and Diagnostic Laboratory Immunology
JF - Clinical and Diagnostic Laboratory Immunology
IS - 1
ER -