TY - JOUR
T1 - Regulation of cornifin α expression in the vaginal and uterine epithelium by estrogen and retinoic acid
AU - Jetten, Anton M.
AU - De Luca, Luigi M.
AU - Nelson, Kay
AU - Schroeder, Wanda
AU - Burlingame, Sue
AU - Fujimoto, Wataru
PY - 1996/10/14
Y1 - 1996/10/14
N2 - In this study, we analyze the regulation of the squamous-specific gene, cornifin α, by estrogen and retinoic acid in vaginal and uterine epithelial cells. In ovariectomized animals, the vaginal epithelium consists of a stratified, nonkeratinizing epithelium which changes into a highly-stratified, keratinizing epithelium upon treatment with estradiol. This transition is accompanied by a dramatic induction of the crosslinked envelope precursor, cornifin α. An increase in cornifin mRNA can be detected as early as 3 h after treatment. A similar effect is observed for the synthetic estrogenic agent diethylstilbestrol while other steroid hormones, including testosterone, progesterone or dexamethasone have little effect on cornifin expression. In contrast to the vagina, estradiol induces neither squamous differentiation nor expression of cornifin α in the uterine epithelium. Similar to the action of estradiol, vitamin A-deficiency greatly enhances squamous differentiation and keratinization in the vaginal epithelium. But unlike estradiol, it induces squamous metaplasia in the normally columnar, uterine epithelium, which eventually is replaced by a keratinizing epithelium in severe deficiency. This transition is associated with an induction of cornifin a expression. Immunohistochemical and in situ hybridization analysis localizes cornifin protein and mRNA in the suprabasal layers of the squamous epithelium. Our results demonstrate that estrogen and retinoids play key roles in the regulation of differentiation and cornifin α expression in the uterine and vaginal epithelium.
AB - In this study, we analyze the regulation of the squamous-specific gene, cornifin α, by estrogen and retinoic acid in vaginal and uterine epithelial cells. In ovariectomized animals, the vaginal epithelium consists of a stratified, nonkeratinizing epithelium which changes into a highly-stratified, keratinizing epithelium upon treatment with estradiol. This transition is accompanied by a dramatic induction of the crosslinked envelope precursor, cornifin α. An increase in cornifin mRNA can be detected as early as 3 h after treatment. A similar effect is observed for the synthetic estrogenic agent diethylstilbestrol while other steroid hormones, including testosterone, progesterone or dexamethasone have little effect on cornifin expression. In contrast to the vagina, estradiol induces neither squamous differentiation nor expression of cornifin α in the uterine epithelium. Similar to the action of estradiol, vitamin A-deficiency greatly enhances squamous differentiation and keratinization in the vaginal epithelium. But unlike estradiol, it induces squamous metaplasia in the normally columnar, uterine epithelium, which eventually is replaced by a keratinizing epithelium in severe deficiency. This transition is associated with an induction of cornifin a expression. Immunohistochemical and in situ hybridization analysis localizes cornifin protein and mRNA in the suprabasal layers of the squamous epithelium. Our results demonstrate that estrogen and retinoids play key roles in the regulation of differentiation and cornifin α expression in the uterine and vaginal epithelium.
KW - Cornifin
KW - Estrogen
KW - Retinoic acid
KW - Squamous differentiation
KW - Uterus
KW - Vagina
KW - Vitamin A
UR - http://www.scopus.com/inward/record.url?scp=0030583601&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030583601&partnerID=8YFLogxK
U2 - 10.1016/0303-7207(96)03871-3
DO - 10.1016/0303-7207(96)03871-3
M3 - Article
C2 - 8912806
AN - SCOPUS:0030583601
SN - 0303-7207
VL - 123
SP - 7
EP - 15
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 1
ER -