Regulation and function of Gα protein subunits in Dictyostelium

A. Kumagai; M. Pupillo; R. Gundersen; R. Miake-Lye; P. N. Devreotes; R. A. Firtel

doi:10.1016/0092-8674(89)90964-1

Regulation and function of G_α protein subunits in Dictyostelium

A. Kumagai, M. Pupillo, R. Gundersen, R. Miake-Lye, P. N. Devreotes, R. A. Firtel

School of Medicine

Research output: Contribution to journal › Article › peer-review

152 Scopus citations

Abstract

We have examined the developmental regulation and function of two G_α protein subunits, G_α1 and G_α2, from Dictyostelium. G_α1 is expressed in vegetative cells through aggregate stages while G_α2 is inducible by cAMP pulses and preferentially expressed in aggregation. Our results suggest that G_α2 encodes the G_α protein subunit associated with the cAMP receptor and mediates all known receptor-activated intracellular signal transduction processes, including chemotaxis and gene regulation. G_α1 appears to function in both the cell cycle and development. Overexpression of G_α1 results in large, multinucleated cells that develop abnormally. The central role that these G_α proteins play in signal transduction processes and in controlling Dictyostelium development is discussed.

Original language	English (US)
Pages (from-to)	265-275
Number of pages	11
Journal	Cell
Volume	57
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(89)90964-1
State	Published - Apr 21 1989

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/0092-8674(89)90964-1

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title = "Regulation and function of Gα protein subunits in Dictyostelium",

abstract = "We have examined the developmental regulation and function of two Gα protein subunits, Gα1 and Gα2, from Dictyostelium. Gα1 is expressed in vegetative cells through aggregate stages while Gα2 is inducible by cAMP pulses and preferentially expressed in aggregation. Our results suggest that Gα2 encodes the Gα protein subunit associated with the cAMP receptor and mediates all known receptor-activated intracellular signal transduction processes, including chemotaxis and gene regulation. Gα1 appears to function in both the cell cycle and development. Overexpression of Gα1 results in large, multinucleated cells that develop abnormally. The central role that these Gα proteins play in signal transduction processes and in controlling Dictyostelium development is discussed.",

author = "A. Kumagai and M. Pupillo and R. Gundersen and R. Miake-Lye and Devreotes, {P. N.} and Firtel, {R. A.}",

note = "Funding Information: We would like to acknowledge Dr. M. Simon for helpful discussions and for support for part of this work. We would like to thank Sandra Mann for supplying a number of the RNA samples. This work was supported by National Institutes of Health grants to P N. D. and Ft. A. F. and by a University of California Systemwide Biotechnology grant to R. A. F The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.",

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doi = "10.1016/0092-8674(89)90964-1",

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AU - Kumagai, A.

AU - Pupillo, M.

AU - Gundersen, R.

AU - Miake-Lye, R.

AU - Devreotes, P. N.

AU - Firtel, R. A.

N1 - Funding Information: We would like to acknowledge Dr. M. Simon for helpful discussions and for support for part of this work. We would like to thank Sandra Mann for supplying a number of the RNA samples. This work was supported by National Institutes of Health grants to P N. D. and Ft. A. F. and by a University of California Systemwide Biotechnology grant to R. A. F The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

PY - 1989/4/21

Y1 - 1989/4/21

N2 - We have examined the developmental regulation and function of two Gα protein subunits, Gα1 and Gα2, from Dictyostelium. Gα1 is expressed in vegetative cells through aggregate stages while Gα2 is inducible by cAMP pulses and preferentially expressed in aggregation. Our results suggest that Gα2 encodes the Gα protein subunit associated with the cAMP receptor and mediates all known receptor-activated intracellular signal transduction processes, including chemotaxis and gene regulation. Gα1 appears to function in both the cell cycle and development. Overexpression of Gα1 results in large, multinucleated cells that develop abnormally. The central role that these Gα proteins play in signal transduction processes and in controlling Dictyostelium development is discussed.

AB - We have examined the developmental regulation and function of two Gα protein subunits, Gα1 and Gα2, from Dictyostelium. Gα1 is expressed in vegetative cells through aggregate stages while Gα2 is inducible by cAMP pulses and preferentially expressed in aggregation. Our results suggest that Gα2 encodes the Gα protein subunit associated with the cAMP receptor and mediates all known receptor-activated intracellular signal transduction processes, including chemotaxis and gene regulation. Gα1 appears to function in both the cell cycle and development. Overexpression of Gα1 results in large, multinucleated cells that develop abnormally. The central role that these Gα proteins play in signal transduction processes and in controlling Dictyostelium development is discussed.

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Regulation and function of G_α protein subunits in Dictyostelium

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