TY - JOUR
T1 - Recovery of protein from tear fluid stored in cellulose sponges
AU - van Agtmaal, Eric J.
AU - van Haeringen, Nicolaas J.
AU - Bloem, Martin W.
AU - Schreurs, Wil H.P.
AU - Saowakontha, Sastri
N1 - Funding Information:
ACKNOWLEDGEMENTS The current investigation was carried out within the scope of the bilateral Thai-Netherlands Nutrition/Health Development Programme in Northeast Thailand with financial support of the Dutch Government and under the name "Nutrition Supplement Co-operation Project" (NSC-project). This study was partly supported by the "Vereniging Blindenbelangen Rotterdam". The authors wish to thank mrs. L. Luyendijk, department of Ophthalmo-Immunology of the Netherlands Ophthalmic Research Institute, for analysis of IgA and LF.
PY - 1987
Y1 - 1987
N2 - Human tear fluid collected in glass capillaries was absorbed in cellulose sponges. After various time intervals the recovery was measured for total protein, amylase (AMY), lysozyme (LZM), immunoglobulin A (IgA) and lactoferrin (LF). After one day storage at 4°C of moistened sponges of at least 92% recovery was observed. Storage for longer periods at 4°C up to seven days showed a decreasing recovery for total protein, AMY and IgA. Tear collection with sponges and storage of wet sponges for maximally one day at 4°C followed by centrifugation or storage of the fluid at-20°C are reliable ways of sample treatment.
AB - Human tear fluid collected in glass capillaries was absorbed in cellulose sponges. After various time intervals the recovery was measured for total protein, amylase (AMY), lysozyme (LZM), immunoglobulin A (IgA) and lactoferrin (LF). After one day storage at 4°C of moistened sponges of at least 92% recovery was observed. Storage for longer periods at 4°C up to seven days showed a decreasing recovery for total protein, AMY and IgA. Tear collection with sponges and storage of wet sponges for maximally one day at 4°C followed by centrifugation or storage of the fluid at-20°C are reliable ways of sample treatment.
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U2 - 10.3109/02713688709025217
DO - 10.3109/02713688709025217
M3 - Article
C2 - 2438087
AN - SCOPUS:0023113716
SN - 0271-3683
VL - 6
SP - 585
EP - 588
JO - Current Eye Research
JF - Current Eye Research
IS - 4
ER -