Reconstitution of acetylcholine receptor from Torpedo californica with highly purified phospholipids: Effect of α-tocopherol, phylloquinone, and other terpenoid quinones

Patricia L. Kilian; Carolyn R. Dunlap; Paul Mueller; Mark A. Schell; Richard L. Huganir; Efraim Racker

doi:10.1016/0006-291X(80)91092-X

Reconstitution of acetylcholine receptor from Torpedo californica with highly purified phospholipids: Effect of α-tocopherol, phylloquinone, and other terpenoid quinones

Patricia L. Kilian, Carolyn R. Dunlap, Paul Mueller, Mark A. Schell, Richard L. Huganir, Efraim Racker

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Acetylcholine receptor from Torpedo californica can be incorporated by the cholate dialysis procedure into liposomes prepared with crude soybean phospholipids (asolectin). Vesicles reconstituted with asolectin depleted of neutral lipids or with a mixture of pure phospholipids, are less active in catalyzing carbamylcholine-sensitive Na⁺ flux. Inclusion of α-tocopherol or certain quinones such as coenzyme Q₁₀ or vitamin K₁ during reconstitution yields vesicles with carbamylcholine-sensitive Na⁺ flux which, under optimal conditions, was considerably higher than that observed with vesicles reconstituted with crude phospholipid mixtures.

Original language	English (US)
Pages (from-to)	409-414
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	93
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(80)91092-X
State	Published - Mar 28 1980
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Kilian, P. L., Dunlap, C. R., Mueller, P., Schell, M. A., Huganir, R. L., & Racker, E. (1980). Reconstitution of acetylcholine receptor from Torpedo californica with highly purified phospholipids: Effect of α-tocopherol, phylloquinone, and other terpenoid quinones. Biochemical and Biophysical Research Communications, 93(2), 409-414. https://doi.org/10.1016/0006-291X(80)91092-X

Reconstitution of acetylcholine receptor from Torpedo californica with highly purified phospholipids: Effect of α-tocopherol, phylloquinone, and other terpenoid quinones. / Kilian, Patricia L.; Dunlap, Carolyn R.; Mueller, Paul et al.
In: Biochemical and Biophysical Research Communications, Vol. 93, No. 2, 28.03.1980, p. 409-414.

Research output: Contribution to journal › Article › peer-review

Kilian, PL, Dunlap, CR, Mueller, P, Schell, MA, Huganir, RL & Racker, E 1980, 'Reconstitution of acetylcholine receptor from Torpedo californica with highly purified phospholipids: Effect of α-tocopherol, phylloquinone, and other terpenoid quinones', Biochemical and Biophysical Research Communications, vol. 93, no. 2, pp. 409-414. https://doi.org/10.1016/0006-291X(80)91092-X

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Reconstitution of acetylcholine receptor from Torpedo californica with highly purified phospholipids: Effect of α-tocopherol, phylloquinone, and other terpenoid quinones

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