Abstract
Acetylcholine receptor from Torpedo californica can be incorporated by the cholate dialysis procedure into liposomes prepared with crude soybean phospholipids (asolectin). Vesicles reconstituted with asolectin depleted of neutral lipids or with a mixture of pure phospholipids, are less active in catalyzing carbamylcholine-sensitive Na+ flux. Inclusion of α-tocopherol or certain quinones such as coenzyme Q10 or vitamin K1 during reconstitution yields vesicles with carbamylcholine-sensitive Na+ flux which, under optimal conditions, was considerably higher than that observed with vesicles reconstituted with crude phospholipid mixtures.
Original language | English (US) |
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Pages (from-to) | 409-414 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 93 |
Issue number | 2 |
DOIs | |
State | Published - Mar 28 1980 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology