Recombination-mediated PCR-directed plasmid construction in vivo in yeast

Kevin R. Oldenburg, Kham T. Vo, Susan Michaelis, Chris Paddon

Research output: Contribution to journalArticlepeer-review

381 Scopus citations


We have extended the technique of PCR-directed recombination in Saccharomyces cerevisiae to develop a simple method for plasmid or gene construction in the absence of suitable restriction sites. The DNA to be cloned is PCR-amplified with 30-40 bp of homology to a linearized yeast plasmid. Co-transformation into yeast results in homologous recombination at a position directed by the PCR oligonucleotides.

Original languageEnglish (US)
Pages (from-to)451-452
Number of pages2
JournalNucleic acids research
Issue number2
StatePublished - 1997

ASJC Scopus subject areas

  • Genetics


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