Abstract
Multiple particle tracking (MPT) was used to study the mechanism of gene delivery with a non-viral DNA delivery system. Movements of hundreds of individual fluorescent PEI/DNA nanocomplexes were tracked through COS-7 cells and their transport properties were quantified by calculating their mean-square displacements (MSD). Average MSDs of complexes did not change significantly over the first 5 h post-transfection. At a time scale of 10 s, MSD values ranged from 0.21 to 0.57 μm2, compared to < 0.32 μm2 for 6kb-sized naked DNA[1]. Velocities calculated for individual particles showed some moved as fast as 0.26 μm/s, while others were almost immobile. Interestingly, 35% of the complexes were transported in a directed fashion 0.5 h after transfection (rather than random diffusion), a phenomenon that could not be detected by other methods such as Fluorescence Recovery After Photobleaching (FRAP). The percentage of cells undergoing directed transport increased to 62% after 5 h. Studies such as these should allow the systematic optimization of the physicochemical properties of non-viral gene carriers for enhanced gene transfection efficacy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 548-549 |
| Number of pages | 2 |
| Journal | Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings |
| Volume | 1 |
| State | Published - Dec 1 2002 |
| Event | Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States Duration: Oct 23 2002 → Oct 26 2002 |
Keywords
- COS-7
- Intracellular
- Particle tracking
- PEI
ASJC Scopus subject areas
- Signal Processing
- Biomedical Engineering
- Computer Vision and Pattern Recognition
- Health Informatics