TY - JOUR
T1 - Real-time non-contact cellular imaging and angiography of human cornea and limbus with common-path full-field/SD OCT
AU - Mazlin, Viacheslav
AU - Xiao, Peng
AU - Scholler, Jules
AU - Irsch, Kristina
AU - Grieve, Kate
AU - Fink, Mathias
AU - Boccara, A. Claude
N1 - Funding Information:
This work was supported by the HELMHOLTZ synergy grant funded by the European Research Council (ERC) (610110) and by a CNRS pre-maturation grant, as well as by the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 709104 (K.I.). We are grateful to Cristina Georgeon and Marie Borderie for assistance with acquiring the slit-lamp and specular microscope images. We thank Michel Paques and Jean-Marie Chassot for valuable discussions.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - In today’s clinics, a cell-resolution view of the cornea can be achieved only with a confocal microscope (IVCM) in contact with the eye. Here, we present a common-path full-field/spectral-domain OCT microscope (FF/SD OCT), which enables cell-detail imaging of the entire ocular surface in humans (central and peripheral cornea, limbus, sclera, tear film) without contact and in real-time. Real-time performance is achieved through rapid axial eye tracking and simultaneous defocusing correction. Images contain cells and nerves, which can be quantified over a millimetric field-of-view, beyond the capability of IVCM and conventional OCT. In the limbus, palisades of Vogt, vessels, and blood flow can be resolved with high contrast without contrast agent injection. The fast imaging speed of 275 frames/s (0.6 billion pixels/s) allows direct monitoring of blood flow dynamics, enabling creation of high-resolution velocity maps. Tear flow velocity and evaporation time can be measured without fluorescein administration.
AB - In today’s clinics, a cell-resolution view of the cornea can be achieved only with a confocal microscope (IVCM) in contact with the eye. Here, we present a common-path full-field/spectral-domain OCT microscope (FF/SD OCT), which enables cell-detail imaging of the entire ocular surface in humans (central and peripheral cornea, limbus, sclera, tear film) without contact and in real-time. Real-time performance is achieved through rapid axial eye tracking and simultaneous defocusing correction. Images contain cells and nerves, which can be quantified over a millimetric field-of-view, beyond the capability of IVCM and conventional OCT. In the limbus, palisades of Vogt, vessels, and blood flow can be resolved with high contrast without contrast agent injection. The fast imaging speed of 275 frames/s (0.6 billion pixels/s) allows direct monitoring of blood flow dynamics, enabling creation of high-resolution velocity maps. Tear flow velocity and evaporation time can be measured without fluorescein administration.
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U2 - 10.1038/s41467-020-15792-x
DO - 10.1038/s41467-020-15792-x
M3 - Article
C2 - 32313067
AN - SCOPUS:85083773590
SN - 2041-1723
VL - 11
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 1868
ER -