TY - JOUR
T1 - Real-Time Monitoring of a Protein Biomarker
AU - Parolo, Claudio
AU - Idili, Andrea
AU - Ortega, Gabriel
AU - Csordas, Andrew
AU - Hsu, Alex
AU - Arroyo-Currás, Netzahualcoýotl
AU - Yang, Qin
AU - Ferguson, Brian Scott
AU - Wang, Jinpeng
AU - Plaxco, Kevin W.
N1 - Funding Information:
This work was funded by Grant EB022015 from the National Institutes of Health. C.P. thanks the Generalitat de Catalunya for the Beatriu de Pino’s fellowship (2014 BP_A 00068).
Publisher Copyright:
Copyright © 2020 American Chemical Society.
PY - 2020/7/24
Y1 - 2020/7/24
N2 - The ability to monitor protein biomarkers continuously and in real-time would significantly advance the precision of medicine. Current protein-detection techniques, however, including ELISA and lateral flow assays, provide only time-delayed, single-time-point measurements, limiting their ability to guide prompt responses to rapidly evolving, life-threatening conditions. In response, here we present an electrochemical aptamer-based sensor (EAB) that supports high-frequency, real-time biomarker measurements. Specifically, we have developed an electrochemical, aptamer-based (EAB) sensor against Neutrophil Gelatinase-Associated Lipocalin (NGAL), a protein that, if present in urine at levels above a threshold value, is indicative of acute renal/kidney injury (AKI). When deployed inside a urinary catheter, the resulting reagentless, wash-free sensor supports real-time, high-frequency monitoring of clinically relevant NGAL concentrations over the course of hours. By providing an "early warning system", the ability to measure levels of diagnostically relevant proteins such as NGAL in real-time could fundamentally change how we detect, monitor, and treat many important diseases.
AB - The ability to monitor protein biomarkers continuously and in real-time would significantly advance the precision of medicine. Current protein-detection techniques, however, including ELISA and lateral flow assays, provide only time-delayed, single-time-point measurements, limiting their ability to guide prompt responses to rapidly evolving, life-threatening conditions. In response, here we present an electrochemical aptamer-based sensor (EAB) that supports high-frequency, real-time biomarker measurements. Specifically, we have developed an electrochemical, aptamer-based (EAB) sensor against Neutrophil Gelatinase-Associated Lipocalin (NGAL), a protein that, if present in urine at levels above a threshold value, is indicative of acute renal/kidney injury (AKI). When deployed inside a urinary catheter, the resulting reagentless, wash-free sensor supports real-time, high-frequency monitoring of clinically relevant NGAL concentrations over the course of hours. By providing an "early warning system", the ability to measure levels of diagnostically relevant proteins such as NGAL in real-time could fundamentally change how we detect, monitor, and treat many important diseases.
KW - acute kidney injury
KW - aptamer
KW - electrochemical sensor
KW - neutrophil gelatinase-associated lipocalin
KW - real-time protein monitoring
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U2 - 10.1021/acssensors.0c01085
DO - 10.1021/acssensors.0c01085
M3 - Article
C2 - 32619092
AN - SCOPUS:85088607584
SN - 2379-3694
VL - 5
SP - 1877
EP - 1881
JO - ACS Sensors
JF - ACS Sensors
IS - 7
ER -