Real-time detection of neurite outgrowth using microfluidic device

Samhwan Kim; Jongmoon Jang; Hongsoo Choi; Cheil Moon

doi:10.1117/12.2018667

Real-time detection of neurite outgrowth using microfluidic device

Samhwan Kim, Jongmoon Jang, Hongsoo Choi, Cheil Moon

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

We developed a simple method for real-time detection of the neurite outgrowth using microfluidic device. Our microfluidic device contains three compartmentalized channels which are for cell seeding, hydrogel and growth factors. Collagen gel is filled in the middle channel and pheochromocytoma (PC12) cells are seeded in the left channel. To induce differentiation of PC12 cells, 50 ng/ml to1000 ng/ml of nerve growth factor (NGF) is introduced into the right channel. After three days of NGF treatment, PC12 cells begin to extend neurites and formed neurite network from sixth day. Quantification of neurite outgrowth is analyzed by measuring the total area of neurites. On sixth day, the area is doubled compared to the area on third day and increases by 20 times on ninth day.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Volume	8879
DOIs	https://doi.org/10.1117/12.2018667
State	Published - 2013
Externally published	Yes
Event	SPIE Nano-Bio Sensing, Imaging, and Spectroscopy - Jeju, Korea, Republic of Duration: Feb 20 2013 → Feb 23 2013

Other

Other	SPIE Nano-Bio Sensing, Imaging, and Spectroscopy
Country/Territory	Korea, Republic of
City	Jeju
Period	2/20/13 → 2/23/13

Keywords

Microfluidic device
Nerve growth factor
Neurite outgrowth

ASJC Scopus subject areas

Applied Mathematics
Computer Science Applications
Electrical and Electronic Engineering
Electronic, Optical and Magnetic Materials
Condensed Matter Physics

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10.1117/12.2018667

Cite this

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title = "Real-time detection of neurite outgrowth using microfluidic device",

abstract = "We developed a simple method for real-time detection of the neurite outgrowth using microfluidic device. Our microfluidic device contains three compartmentalized channels which are for cell seeding, hydrogel and growth factors. Collagen gel is filled in the middle channel and pheochromocytoma (PC12) cells are seeded in the left channel. To induce differentiation of PC12 cells, 50 ng/ml to1000 ng/ml of nerve growth factor (NGF) is introduced into the right channel. After three days of NGF treatment, PC12 cells begin to extend neurites and formed neurite network from sixth day. Quantification of neurite outgrowth is analyzed by measuring the total area of neurites. On sixth day, the area is doubled compared to the area on third day and increases by 20 times on ninth day.",

keywords = "Microfluidic device, Nerve growth factor, Neurite outgrowth",

author = "Samhwan Kim and Jongmoon Jang and Hongsoo Choi and Cheil Moon",

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note = "SPIE Nano-Bio Sensing, Imaging, and Spectroscopy ; Conference date: 20-02-2013 Through 23-02-2013",

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TY - GEN

T1 - Real-time detection of neurite outgrowth using microfluidic device

AU - Kim, Samhwan

AU - Jang, Jongmoon

AU - Choi, Hongsoo

AU - Moon, Cheil

PY - 2013

Y1 - 2013

N2 - We developed a simple method for real-time detection of the neurite outgrowth using microfluidic device. Our microfluidic device contains three compartmentalized channels which are for cell seeding, hydrogel and growth factors. Collagen gel is filled in the middle channel and pheochromocytoma (PC12) cells are seeded in the left channel. To induce differentiation of PC12 cells, 50 ng/ml to1000 ng/ml of nerve growth factor (NGF) is introduced into the right channel. After three days of NGF treatment, PC12 cells begin to extend neurites and formed neurite network from sixth day. Quantification of neurite outgrowth is analyzed by measuring the total area of neurites. On sixth day, the area is doubled compared to the area on third day and increases by 20 times on ninth day.

AB - We developed a simple method for real-time detection of the neurite outgrowth using microfluidic device. Our microfluidic device contains three compartmentalized channels which are for cell seeding, hydrogel and growth factors. Collagen gel is filled in the middle channel and pheochromocytoma (PC12) cells are seeded in the left channel. To induce differentiation of PC12 cells, 50 ng/ml to1000 ng/ml of nerve growth factor (NGF) is introduced into the right channel. After three days of NGF treatment, PC12 cells begin to extend neurites and formed neurite network from sixth day. Quantification of neurite outgrowth is analyzed by measuring the total area of neurites. On sixth day, the area is doubled compared to the area on third day and increases by 20 times on ninth day.

KW - Microfluidic device

KW - Nerve growth factor

KW - Neurite outgrowth

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DO - 10.1117/12.2018667

M3 - Conference contribution

AN - SCOPUS:84883765567

SN - 9780819493040

VL - 8879

BT - Proceedings of SPIE - The International Society for Optical Engineering

T2 - SPIE Nano-Bio Sensing, Imaging, and Spectroscopy

Y2 - 20 February 2013 through 23 February 2013

ER -

Real-time detection of neurite outgrowth using microfluidic device

Abstract

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Keywords

ASJC Scopus subject areas

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