@article{f6f778e8cab247979e75277dc59093d4,
title = "Real-time crystallographic studies of the adenine riboswitch using an X-ray free-electron laser",
abstract = "Structures of the four reaction states of the adenine riboswitch aptamer domain, including a transient intermediate state were solved by serial femtosecond crystallography. The structures not only demonstrate the use of X-ray free-electron lasers for RNA crystallography but have also proven that transient states can be determined in real time by mix-and-inject crystallography. These results illustrate the structural basis for the ligand-induced conformational changes associated with the molecular {\textquoteleft}switch{\textquoteright}.",
keywords = "RNA, X-ray free-electron laser, adenine riboswitch, diffusive mixing, lattice conversion, phase transition, reaction intermediate, serial femtosecond crystallography, time-resolved crystallography",
author = "Stagno, {Jason R.} and Bhandari, {Yuba R.} and Conrad, {Chelsie E.} and Yu Liu and Wang, {Yun Xing}",
note = "Funding Information: This work was supported in part by the NIH Intramural Research Programs of NCI and CIT and by the US Department of Energy, Office of Biological and Environmental Research, under Contract DE-AC02-06CH11357. Portions of this research were carried out at the LCLS, a National User Facility operated by Stanford University on behalf of the US Department of Energy, Office of Basic Energy Sciences. The CXI instrument was funded by the LCLS Ultrafast Science Instruments (LUSI) project funded by the US Department of Energy, Office of Basic Energy Sciences. Use of the LCLS, SLAC National Accelerator Laboratory, is supported by the US Department of Energy, Office of Science, Office of Basic Energy Sciences under Contract No. DE-AC02-76SF00515. Publisher Copyright: Published 2017. This article is a U.S. Government work and is in the public domain in the USA.",
year = "2017",
month = oct,
doi = "10.1111/febs.14110",
language = "English (US)",
volume = "284",
pages = "3374--3380",
journal = "FEBS Journal",
issn = "1742-464X",
publisher = "Wiley-Blackwell",
number = "20",
}