Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange

Yidan Ding; Jiao Li; Jhon Ralph Enterina; Yi Shen; Issan Zhang; Paul H. Tewson; Gary C H Mo; Jin Zhang; Anne Marie Quinn; Thomas E. Hughes; Dusica Maysinger; Spencer C. Alford; Yan Zhang; Robert E. Campbell

doi:10.1038/nmeth.3261

Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange

Yidan Ding
, Jiao Li
, Jhon Ralph Enterina
, Yi Shen
, Issan Zhang
, Paul H. Tewson
, Gary C H Mo
, Jin Zhang
, Anne Marie Quinn
, Thomas E. Hughes
, Dusica Maysinger
, Spencer C. Alford
, Yan Zhang
, Robert E. Campbell

School of Medicine

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca 2+ concentration dynamics and other second-messenger signaling activities.

Original language	English (US)
Pages (from-to)	195-198
Number of pages	4
Journal	Nature Methods
Volume	12
Issue number	3
DOIs	https://doi.org/10.1038/nmeth.3261
State	Published - Feb 26 2015

ASJC Scopus subject areas

Biotechnology
Molecular Biology
Biochemistry
Cell Biology

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10.1038/nmeth.3261

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title = "Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange",

abstract = "We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca 2+ concentration dynamics and other second-messenger signaling activities.",

author = "Yidan Ding and Jiao Li and Enterina, \{Jhon Ralph\} and Yi Shen and Issan Zhang and Tewson, \{Paul H.\} and Mo, \{Gary C H\} and Jin Zhang and Quinn, \{Anne Marie\} and Hughes, \{Thomas E.\} and Dusica Maysinger and Alford, \{Spencer C.\} and Yan Zhang and Campbell, \{Robert E.\}",

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journal = "Nature Methods",

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AU - Ding, Yidan

AU - Li, Jiao

AU - Enterina, Jhon Ralph

AU - Shen, Yi

AU - Zhang, Issan

AU - Tewson, Paul H.

AU - Mo, Gary C H

AU - Zhang, Jin

AU - Quinn, Anne Marie

AU - Hughes, Thomas E.

AU - Maysinger, Dusica

AU - Alford, Spencer C.

AU - Zhang, Yan

AU - Campbell, Robert E.

PY - 2015/2/26

Y1 - 2015/2/26

N2 - We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca 2+ concentration dynamics and other second-messenger signaling activities.

AB - We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca 2+ concentration dynamics and other second-messenger signaling activities.

UR - https://www.scopus.com/pages/publications/84923845791

UR - https://www.scopus.com/pages/publications/84923845791#tab=citedBy

U2 - 10.1038/nmeth.3261

DO - 10.1038/nmeth.3261

M3 - Article

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AN - SCOPUS:84923845791

SN - 1548-7091

VL - 12

SP - 195

EP - 198

JO - Nature Methods

JF - Nature Methods

IS - 3

ER -

Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange

Abstract

ASJC Scopus subject areas

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