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Ratiometric autofluorescence lifetime imaging system standardization and application for head and neck cancer

  • Peter A. Pellionisz
  • , Yong Hu
  • , Andy Moon
  • , Khuzaima Rangwalla
  • , Jake Pensa
  • , Maie A. St John

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Dynamic optical contrast imaging (DOCI) is a novel optical imaging technology that rapidly generates image contrast from measurements of aggregate endogenous fluorescence lifetime in a clinically meaningful field of view. Recently, our use of this system in both human ex-vivo and in-vivo specimens generated statistically significant contrast between tumor and adjacent normal tissue in biopsies taken from patients undergoing surgery for head and neck squamous cell carcinoma and primary hyperthyroidism. In this work we evaluated the components and resolution of our next-generation DOCI system. We also standardized the quantitative output of our system against the fluorescence lifetime values of three dye standards with monoexponential decay using a commercial Leica two-photon fluorescence lifetime imaging microscope. Significantly, our system continued to demonstrate clinically meaningful contrast between tissue samples with multiexponential decay in near real-time.

Original languageEnglish (US)
Title of host publicationOptics in Health Care and Biomedical Optics IX
EditorsQingming Luo, Qingming Luo, Xingde Li, Ying Gu, Yuguo Tang, Dan Zhu
PublisherSPIE
ISBN (Electronic)9781510630970
DOIs
StatePublished - 2019
Externally publishedYes
EventOptics in Health Care and Biomedical Optics IX 2019 - Hangzhou, China
Duration: Oct 21 2019Oct 23 2019

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume11190
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceOptics in Health Care and Biomedical Optics IX 2019
Country/TerritoryChina
CityHangzhou
Period10/21/1910/23/19

Keywords

  • diagnostic imaging
  • fluorescence lifetime
  • head and neck cancer

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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