Rat thyroid peroxidase (TPO) biosynthesis in vitro: Studies using antiserum to porcine TPO

David S. Cooper; Farahe Maloof; E. Chester Ridgway

doi:10.1080/07435808709023659

Rat thyroid peroxidase (TPO) biosynthesis in vitro: Studies using antiserum to porcine TPO

David S. Cooper, Farahe Maloof, E. Chester Ridgway

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5 Scopus citations

Abstract

Using partially purified trypsin-solubilized porcine thyroid peroxidase (TPO), an antiserum was developed which inhibited enzyme activity in vitro, and which crossreacted with partially purified rat TPO. Using rat thyroid minces, this antiserum was utilized to detect radiolabeled TPO in vitro. Newly synthesized rat TPO was observed in the presence of TSH (10 mU/ml), an effect which was completely blocked by co-incubation with cycloheximide. TPO biosynthesis was detected within 6 h of incubation, consistent within a rapid effect of TSH on thyroidal protein biosynthesis.

Original language	English (US)
Pages (from-to)	15-29
Number of pages	15
Journal	Endocrine Research
Volume	13
Issue number	1
DOIs	https://doi.org/10.1080/07435808709023659
State	Published - Jan 1987
Externally published	Yes

ASJC Scopus subject areas

Endocrinology

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10.1080/07435808709023659

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title = "Rat thyroid peroxidase (TPO) biosynthesis in vitro: Studies using antiserum to porcine TPO",

abstract = "Using partially purified trypsin-solubilized porcine thyroid peroxidase (TPO), an antiserum was developed which inhibited enzyme activity in vitro, and which crossreacted with partially purified rat TPO. Using rat thyroid minces, this antiserum was utilized to detect radiolabeled TPO in vitro. Newly synthesized rat TPO was observed in the presence of TSH (10 mU/ml), an effect which was completely blocked by co-incubation with cycloheximide. TPO biosynthesis was detected within 6 h of incubation, consistent within a rapid effect of TSH on thyroidal protein biosynthesis.",

author = "Cooper, {David S.} and Farahe Maloof and Ridgway, {E. Chester}",

year = "1987",

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AU - Maloof, Farahe

AU - Ridgway, E. Chester

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AB - Using partially purified trypsin-solubilized porcine thyroid peroxidase (TPO), an antiserum was developed which inhibited enzyme activity in vitro, and which crossreacted with partially purified rat TPO. Using rat thyroid minces, this antiserum was utilized to detect radiolabeled TPO in vitro. Newly synthesized rat TPO was observed in the presence of TSH (10 mU/ml), an effect which was completely blocked by co-incubation with cycloheximide. TPO biosynthesis was detected within 6 h of incubation, consistent within a rapid effect of TSH on thyroidal protein biosynthesis.

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Rat thyroid peroxidase (TPO) biosynthesis in vitro: Studies using antiserum to porcine TPO

Abstract

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