Abstract
Tell it where to go: Rapamycin induced the relocation of an FRB-fused protein of interest (POI) to the plasma membrane (labeled with the fusion protein GAIs-FKBP-C2(LACT)) to activate a signaling event (see picture). Subsequent treatment with a gibberellic acid ester led to the relocation of the whole GAIs-FKBP-C2(LACT)/rapamycin/FRB-POI complex to the Tom20-GID1-labeled mitochondria with the termination of POI-dependent signaling.
Original language | English (US) |
---|---|
Pages (from-to) | 6450-6454 |
Number of pages | 5 |
Journal | Angewandte Chemie - International Edition |
Volume | 52 |
Issue number | 25 |
DOIs | |
State | Published - Jun 17 2013 |
Externally published | Yes |
Keywords
- cell signaling
- chemically inducible dimerization
- enzymes
- phosopholipids
- reversible perturbation
ASJC Scopus subject areas
- Catalysis
- Chemistry(all)