Rapidly reversible manipulation of molecular activity with dual chemical dimerizers

Yu Chun Lin; Yuta Nihongaki; Tzu Yu Liu; Shiva Razavi; Moritoshi Sato; Takanari Inoue

doi:10.1002/anie.201301219

Rapidly reversible manipulation of molecular activity with dual chemical dimerizers

Yu Chun Lin, Yuta Nihongaki, Tzu Yu Liu, Shiva Razavi, Moritoshi Sato, Takanari Inoue

School of Medicine

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Tell it where to go: Rapamycin induced the relocation of an FRB-fused protein of interest (POI) to the plasma membrane (labeled with the fusion protein GAIs-FKBP-C2(LACT)) to activate a signaling event (see picture). Subsequent treatment with a gibberellic acid ester led to the relocation of the whole GAIs-FKBP-C2(LACT)/rapamycin/FRB-POI complex to the Tom20-GID1-labeled mitochondria with the termination of POI-dependent signaling.

Original language	English (US)
Pages (from-to)	6450-6454
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	52
Issue number	25
DOIs	https://doi.org/10.1002/anie.201301219
State	Published - Jun 17 2013

Keywords

cell signaling
chemically inducible dimerization
enzymes
phosopholipids
reversible perturbation

ASJC Scopus subject areas

Catalysis
Chemistry(all)

Access to Document

10.1002/anie.201301219

Cite this

@article{471f0b8f0e014f189a5ad0eabbc0da2d,

title = "Rapidly reversible manipulation of molecular activity with dual chemical dimerizers",

abstract = "Tell it where to go: Rapamycin induced the relocation of an FRB-fused protein of interest (POI) to the plasma membrane (labeled with the fusion protein GAIs-FKBP-C2(LACT)) to activate a signaling event (see picture). Subsequent treatment with a gibberellic acid ester led to the relocation of the whole GAIs-FKBP-C2(LACT)/rapamycin/FRB-POI complex to the Tom20-GID1-labeled mitochondria with the termination of POI-dependent signaling.",

keywords = "cell signaling, chemically inducible dimerization, enzymes, phosopholipids, reversible perturbation",

author = "Lin, {Yu Chun} and Yuta Nihongaki and Liu, {Tzu Yu} and Shiva Razavi and Moritoshi Sato and Takanari Inoue",

year = "2013",

month = jun,

day = "17",

doi = "10.1002/anie.201301219",

language = "English (US)",

volume = "52",

pages = "6450--6454",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley and Sons Ltd",

number = "25",

}

TY - JOUR

T1 - Rapidly reversible manipulation of molecular activity with dual chemical dimerizers

AU - Lin, Yu Chun

AU - Nihongaki, Yuta

AU - Liu, Tzu Yu

AU - Razavi, Shiva

AU - Sato, Moritoshi

AU - Inoue, Takanari

PY - 2013/6/17

Y1 - 2013/6/17

N2 - Tell it where to go: Rapamycin induced the relocation of an FRB-fused protein of interest (POI) to the plasma membrane (labeled with the fusion protein GAIs-FKBP-C2(LACT)) to activate a signaling event (see picture). Subsequent treatment with a gibberellic acid ester led to the relocation of the whole GAIs-FKBP-C2(LACT)/rapamycin/FRB-POI complex to the Tom20-GID1-labeled mitochondria with the termination of POI-dependent signaling.

AB - Tell it where to go: Rapamycin induced the relocation of an FRB-fused protein of interest (POI) to the plasma membrane (labeled with the fusion protein GAIs-FKBP-C2(LACT)) to activate a signaling event (see picture). Subsequent treatment with a gibberellic acid ester led to the relocation of the whole GAIs-FKBP-C2(LACT)/rapamycin/FRB-POI complex to the Tom20-GID1-labeled mitochondria with the termination of POI-dependent signaling.

KW - cell signaling

KW - chemically inducible dimerization

KW - enzymes

KW - phosopholipids

KW - reversible perturbation

UR - http://www.scopus.com/inward/record.url?scp=84878915820&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878915820&partnerID=8YFLogxK

U2 - 10.1002/anie.201301219

DO - 10.1002/anie.201301219

M3 - Article

C2 - 23649661

AN - SCOPUS:84878915820

SN - 1433-7851

VL - 52

SP - 6450

EP - 6454

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 25

ER -

Rapidly reversible manipulation of molecular activity with dual chemical dimerizers

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this