Rapid liquid-liquid extraction of cocaine from urine for gas chromatographic-mass spectrometric analysis

Diana Garside, Bruce A. Goldberger, Kenzie L. Preston, Edward J. Cone

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

A novel, simple and economic liquid-liquid extraction method for isolating cocaine from urine was developed utilizing gas chromatography-mass spectrometry (GC-MS) for analysis and quantification. The use of a single nonpolar organic solvent allowed only nonpolar analytes to be extracted from the biological fluid, and consequently, no derivatization step was necessary before CC-MS analysis. Large numbers of specimens (>60) can be extracted in approximately 3 h with this procedure. The method is highly precise (C.V. <7%), accurate (>98%), sensitive (limit of detection of 5 ng/ml) and has a mean recovery of 48.8%.

Original languageEnglish (US)
Pages (from-to)61-65
Number of pages5
JournalJournal of Chromatography B: Biomedical Applications
Volume692
Issue number1
DOIs
StatePublished - Apr 25 1997

Keywords

  • Cocaine

ASJC Scopus subject areas

  • Chemistry(all)

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