Rapid identification of low level hepatitis B-related viral genome in serum

T. J. Liang, K. J. Isselbacher, J. R. Wands

Research output: Contribution to journalArticlepeer-review

48 Scopus citations


A sensitive and specific method has been developed to detect hepatitis B virus (HBV) in serum. The method involves two steps: the capture of viral genome from serum using a high affinity IgM monoclonal antibody directed against a common a domain epitope found on the envelope, and the amplification of viral DNA by the polymerase chain reaction (PCR). The amplification is initiated using 'generic' primers derived from the core and pre-core sequences which are highly conserved amongs the hepadnaviruses. This rapid technique detects less than 10 infectious virions and may be useful in the study of individuals with acute and chronic liver disease of unknown etiology.

Original languageEnglish (US)
Pages (from-to)1367-1371
Number of pages5
JournalJournal of Clinical Investigation
Issue number4
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


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