Rapid identification of Cryptococcus neoformans and Cryptococcus gattii by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Lisa R. McTaggart; Eric Lei; Susan E. Richardson; Linda Hoang; Annette Fothergill; Sean X. Zhang

doi:10.1128/JCM.00651-11

Rapid identification of Cryptococcus neoformans and Cryptococcus gattii by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Lisa R. McTaggart, Eric Lei, Susan E. Richardson, Linda Hoang, Annette Fothergill, Sean X. Zhang

School of Medicine

Research output: Contribution to journal › Article › peer-review

75 Scopus citations

Abstract

Compared to DNA sequence analysis, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) correctly identified 100% of Cryptococcus species, distinguishing the notable pathogens Cryptococcus neoformans and C. gattii. Identification was greatly enhanced by supplementing a commercial spectral library with additional entries to account for subspecies variability.

Original language	English (US)
Pages (from-to)	3050-3053
Number of pages	4
Journal	Journal of clinical microbiology
Volume	49
Issue number	8
DOIs	https://doi.org/10.1128/JCM.00651-11
State	Published - Aug 2011

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.00651-11

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abstract = "Compared to DNA sequence analysis, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) correctly identified 100% of Cryptococcus species, distinguishing the notable pathogens Cryptococcus neoformans and C. gattii. Identification was greatly enhanced by supplementing a commercial spectral library with additional entries to account for subspecies variability.",

author = "McTaggart, {Lisa R.} and Eric Lei and Richardson, {Susan E.} and Linda Hoang and Annette Fothergill and Zhang, {Sean X.}",

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AU - Richardson, Susan E.

AU - Hoang, Linda

AU - Fothergill, Annette

AU - Zhang, Sean X.

PY - 2011/8

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N2 - Compared to DNA sequence analysis, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) correctly identified 100% of Cryptococcus species, distinguishing the notable pathogens Cryptococcus neoformans and C. gattii. Identification was greatly enhanced by supplementing a commercial spectral library with additional entries to account for subspecies variability.

AB - Compared to DNA sequence analysis, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) correctly identified 100% of Cryptococcus species, distinguishing the notable pathogens Cryptococcus neoformans and C. gattii. Identification was greatly enhanced by supplementing a commercial spectral library with additional entries to account for subspecies variability.

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Rapid identification of Cryptococcus neoformans and Cryptococcus gattii by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Abstract

ASJC Scopus subject areas

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