TY - JOUR
T1 - Raman spectroscopic sensing of carbonate intercalation in breast microcalcifications at stereotactic biopsy
AU - Sathyavathi, R.
AU - Saha, Anushree
AU - Soares, Jaqueline S.
AU - Spegazzini, Nicolas
AU - McGee, Sasha
AU - Rao Dasari, Ramachandra
AU - Fitzmaurice, Maryann
AU - Barman, Ishan
N1 - Funding Information:
This research was funded by the National Institute of Biomedical Imaging and Bioengineering (9P41EB015871–27A1), the JHU Whiting School of Engineering Start up Funds and the National Cancer Institute (R01-CA140288). We would like to acknowledge the use of the Leica SCN400 Slide Scanner in the Genetics Department Imaging Facility at Case Western Reserve University made available through a NIH National Center for Research Resources Shared Instrumentation Grant (1S10RR031845). The authors are also grateful for the invaluable efforts of Drs. Nina Klein & Donna Plecha at University Hospitals Case Medical Center, who performed the breast biopsy procedures. J.S.S. wishes to thank the support from the Brazilian agency CNPq.
PY - 2015/4/30
Y1 - 2015/4/30
N2 - Microcalcifications are an early mammographic sign of breast cancer and frequent target for stereotactic biopsy. Despite their indisputable value, microcalcifications, particularly of the type II variety that are comprised of calcium hydroxyapatite deposits, remain one of the least understood disease markers. Here we employed Raman spectroscopy to elucidate the relationship between pathogenicity of breast lesions in fresh biopsy cores and composition of type II microcalcifications. Using a chemometric model of chemical-morphological constituents, acquired Raman spectra were translated to characterize chemical makeup of the lesions. We find that increase in carbonate intercalation in the hydroxyapatite lattice can be reliably employed to differentiate benign from malignant lesions, with algorithms based only on carbonate and cytoplasmic protein content exhibiting excellent negative predictive value (93-98%). Our findings highlight the importance of calcium carbonate, an underrated constituent of microcalcifications, as a spectroscopic marker in breast pathology evaluation and pave the way for improved biopsy guidance.
AB - Microcalcifications are an early mammographic sign of breast cancer and frequent target for stereotactic biopsy. Despite their indisputable value, microcalcifications, particularly of the type II variety that are comprised of calcium hydroxyapatite deposits, remain one of the least understood disease markers. Here we employed Raman spectroscopy to elucidate the relationship between pathogenicity of breast lesions in fresh biopsy cores and composition of type II microcalcifications. Using a chemometric model of chemical-morphological constituents, acquired Raman spectra were translated to characterize chemical makeup of the lesions. We find that increase in carbonate intercalation in the hydroxyapatite lattice can be reliably employed to differentiate benign from malignant lesions, with algorithms based only on carbonate and cytoplasmic protein content exhibiting excellent negative predictive value (93-98%). Our findings highlight the importance of calcium carbonate, an underrated constituent of microcalcifications, as a spectroscopic marker in breast pathology evaluation and pave the way for improved biopsy guidance.
UR - https://www.scopus.com/pages/publications/84944107548
UR - https://www.scopus.com/pages/publications/84944107548#tab=citedBy
U2 - 10.1038/srep09907
DO - 10.1038/srep09907
M3 - Article
C2 - 25927331
AN - SCOPUS:84944107548
SN - 2045-2322
VL - 5
JO - Scientific reports
JF - Scientific reports
M1 - 9907
ER -