@article{01dabf195e8145178263a7af5b73a994,
title = "Radioiodinated (aminostyryl)pyridinium (ASP) dyes: New cell membrane probes for labeling mixed leukocytes and lymphocytes for diagnostic imaging",
abstract = "We prepared [125I/131I]iodo-(aminostyryl)pyridinium dyes from tributylstannyl precursors. ASP 7a and 7b labeled leukocytes ex vivo (70-94%) using saline with or without washing plasma from cells, Viability of peripheral blood lymphocytes (PBLs) (dogs, rats) and splenic lymphocytes (rats) labeled with 7a and 7b (71-82%) was unchanged after labeling (≤88%). Canine 7b-leukocytes showed higher uptake in inflammatory lesions than did 111In oxine leukocytes. At 3 h, aspirates contained more radioiodine than 111In (1.65:1 to 22:1) and radioiodine was cell bound. ROI measurements (3 h) gave abscess to contralateral knee ratios of 12.3 and 10.6 for 131I-7b vs. 4.8 and 2.3 for 111In-oxine.",
keywords = "(Aminostyryl)pyridinium dyes, Inflammation imaging, Leukocyte labeling, Lymphocyte labeling, Radioiodinated cell membrane probes",
author = "Carol Lambert and Mease, {Ronnie C.} and Lee Avren and Truc Le and Hassan Sabet and McAfee, {John G.}",
note = "Funding Information: This work was supported by NIH grant 5 R01 CA32853. The authors thank Dr. Elliot Rachlin of the University of Utah for mm spectral analysis and Dr. Julia W. Albright and Dr. Terry M. Phillips of the George Washington University Medical Center for helpful discussions. Funding Information: All chemicals used in the syntheses were reagent grade and purchased from Aldrich Chemical Co. (Milwaukee, WI). Nuclear magnetic resonance (NMR) spectra were obtained using a Bruker AC-P 300 IT-NMR spectrometer. Elemental analyses were performed by Atlantic Microlab (Norcross, GA). High-resolution mass spectrometry was performed at the University of Utah Mass Spectrometry Laboratory (supported by NSF grant CHE-9002690 and the University of Utah Institutional Funds Committee) on a Finnigan MAT 95 high-resolution mass spectrometer using a cesium gun for liquid SIMS (secondary ion mass spectrometry). The HPLC purification of tributyltin compounds prior to radioiodination was carried out using a Waters Model 625 LC system with a Waters 490E UV/VIS detector set for simultaneous detection at 280 nm and 575 nm. Radio-HPLC was carried out with a Gilson Model 302 HPLC pump, a Gilson Model 111 UV detector set at 280 nm, and a Beckman Model 170 radioisotope detector. Diluent C is a cell-labeling medium produced by Zynaxis Cell Science (Malvern, PA). Lympholyte-Rat is a product of Cedar Lane Laboratories (Ontario, Canada). Isolymph is a product of Gallard-Schlesinger Industries (Carle Place, NY). Histopaque is a product of Sigma Diagnostics (St. Louis, MO). The acetate-citrate-dextrose (ACD) solution was purchased from B&B/Scott Laboratories (Fiskeville, RI). Radiolabeled cell preparations were counted in a Capintec CRC-1OR dose calibrator. Images were obtained on an Ohio Nuclear Technicare 410 gamma camera and processed with an ADAC 33000 computer. Whole blood, plasma, and knee joint aspirate samples were counted in a LKB 1282 Compugamma CS universal gamma counter. Animal studies were conducted with the approval of the GWUMC Institutional Care and Use Committee. Dogs were anesthetized using Innovarvet (0.1 mg/kg) and pentobarbital (30 mg/kg) during all imaging procedures.",
year = "1996",
month = may,
doi = "10.1016/0969-8051(95)02101-9",
language = "English (US)",
volume = "23",
pages = "417--427",
journal = "Nuclear Medicine and Biology",
issn = "0969-8051",
publisher = "Elsevier Inc.",
number = "4",
}