R-spondin1 synergizes with Wnt3A in inducing osteoblast differentiation and osteoprotegerin expression

Wenyan Lu; Kyung Ah Kim; Jianzhong Liu; Arie Abo; Xu Feng; Xu Cao; Yonghe Li

doi:10.1016/j.febslet.2008.01.035

R-spondin1 synergizes with Wnt3A in inducing osteoblast differentiation and osteoprotegerin expression

Wenyan Lu
, Kyung Ah Kim
, Jianzhong Liu
, Arie Abo
, Xu Feng
, Xu Cao
, Yonghe Li

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

R-spondins are a new group of Wnt/β-catenin signaling agonists, however, the role of these proteins in bone remains unclear. We reported herein that R-sponin1 (Rspo1) acted synergistically with Wnt3A to activate Wnt/β-catenin signaling in the uncommitted mesenchymal C2C12 cells. Furthermore, we found that Rspo1 at concentrations as low as 10 ng/ml synergized strongly with Wnt3A to induce C2C12 osteoblastic differentiation and osteoprotegerin expression. These events were blocked by Wnt/β-catenin signaling antagonist Dickkopf-1. Finally, we demonstrated that Rspo1 synergized with Wnt3A to induce primary mouse osteoblast differentiation. Together, these findings suggest that Rpos1 may play an important role in bone remodeling.

Original language	English (US)
Pages (from-to)	643-650
Number of pages	8
Journal	FEBS Letters
Volume	582
Issue number	5
DOIs	https://doi.org/10.1016/j.febslet.2008.01.035
State	Published - Mar 5 2008
Externally published	Yes

Keywords

Osteoblast differentiation
Osteoprotegerin
R-spondin1
Wnt signaling
Wnt3A

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2008.01.035

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@article{bda8e28895f6464bb7648c3d3c8efa57,

title = "R-spondin1 synergizes with Wnt3A in inducing osteoblast differentiation and osteoprotegerin expression",

abstract = "R-spondins are a new group of Wnt/β-catenin signaling agonists, however, the role of these proteins in bone remains unclear. We reported herein that R-sponin1 (Rspo1) acted synergistically with Wnt3A to activate Wnt/β-catenin signaling in the uncommitted mesenchymal C2C12 cells. Furthermore, we found that Rspo1 at concentrations as low as 10 ng/ml synergized strongly with Wnt3A to induce C2C12 osteoblastic differentiation and osteoprotegerin expression. These events were blocked by Wnt/β-catenin signaling antagonist Dickkopf-1. Finally, we demonstrated that Rspo1 synergized with Wnt3A to induce primary mouse osteoblast differentiation. Together, these findings suggest that Rpos1 may play an important role in bone remodeling.",

keywords = "Osteoblast differentiation, Osteoprotegerin, R-spondin1, Wnt signaling, Wnt3A",

author = "Wenyan Lu and Kim, \{Kyung Ah\} and Jianzhong Liu and Arie Abo and Xu Feng and Xu Cao and Yonghe Li",

note = "Funding Information: This work was supported in part by a grant from the American Heart Association (0330118N) to Y.L. We are grateful to Dr. Gail Johnson for providing the GST-E-cadherin cDNA.",

year = "2008",

month = mar,

day = "5",

doi = "10.1016/j.febslet.2008.01.035",

language = "English (US)",

volume = "582",

pages = "643--650",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "5",

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TY - JOUR

T1 - R-spondin1 synergizes with Wnt3A in inducing osteoblast differentiation and osteoprotegerin expression

AU - Lu, Wenyan

AU - Kim, Kyung Ah

AU - Liu, Jianzhong

AU - Abo, Arie

AU - Feng, Xu

AU - Cao, Xu

AU - Li, Yonghe

N1 - Funding Information: This work was supported in part by a grant from the American Heart Association (0330118N) to Y.L. We are grateful to Dr. Gail Johnson for providing the GST-E-cadherin cDNA.

PY - 2008/3/5

Y1 - 2008/3/5

N2 - R-spondins are a new group of Wnt/β-catenin signaling agonists, however, the role of these proteins in bone remains unclear. We reported herein that R-sponin1 (Rspo1) acted synergistically with Wnt3A to activate Wnt/β-catenin signaling in the uncommitted mesenchymal C2C12 cells. Furthermore, we found that Rspo1 at concentrations as low as 10 ng/ml synergized strongly with Wnt3A to induce C2C12 osteoblastic differentiation and osteoprotegerin expression. These events were blocked by Wnt/β-catenin signaling antagonist Dickkopf-1. Finally, we demonstrated that Rspo1 synergized with Wnt3A to induce primary mouse osteoblast differentiation. Together, these findings suggest that Rpos1 may play an important role in bone remodeling.

AB - R-spondins are a new group of Wnt/β-catenin signaling agonists, however, the role of these proteins in bone remains unclear. We reported herein that R-sponin1 (Rspo1) acted synergistically with Wnt3A to activate Wnt/β-catenin signaling in the uncommitted mesenchymal C2C12 cells. Furthermore, we found that Rspo1 at concentrations as low as 10 ng/ml synergized strongly with Wnt3A to induce C2C12 osteoblastic differentiation and osteoprotegerin expression. These events were blocked by Wnt/β-catenin signaling antagonist Dickkopf-1. Finally, we demonstrated that Rspo1 synergized with Wnt3A to induce primary mouse osteoblast differentiation. Together, these findings suggest that Rpos1 may play an important role in bone remodeling.

KW - Osteoblast differentiation

KW - Osteoprotegerin

KW - R-spondin1

KW - Wnt signaling

KW - Wnt3A

UR - https://www.scopus.com/pages/publications/39649102756

UR - https://www.scopus.com/pages/publications/39649102756#tab=citedBy

U2 - 10.1016/j.febslet.2008.01.035

DO - 10.1016/j.febslet.2008.01.035

M3 - Article

C2 - 18242177

AN - SCOPUS:39649102756

SN - 0014-5793

VL - 582

SP - 643

EP - 650

JO - FEBS Letters

JF - FEBS Letters

IS - 5

ER -

R-spondin1 synergizes with Wnt3A in inducing osteoblast differentiation and osteoprotegerin expression

Abstract

Keywords

ASJC Scopus subject areas

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