Quantitative 1H NMR spectroscopy

Santosh Kumar Bharti, Raja Roy

Research output: Contribution to journalReview articlepeer-review

353 Scopus citations


This review illustrates the need to use nuclear magnetic resonance (NMR) spectroscopy for the quantitative analysis of small molecules in their crude forms and in mixtures.We provide the basic concepts of quantitative NMR (qNMR), a brief description of important acquisition and processing parameters responsible for obtaining high-quality, reproducible NMR spectra in order to maximize accuracy, and the latest referencing techniques used for quantitative analysis.We also describe methods that are used for quantitative analysis including calibration-curve and standard-addition methods. Further, we briefly address validation of qNMR spectroscopy and its major applications in various scientific disciplines.

Original languageEnglish (US)
Pages (from-to)5-26
Number of pages22
JournalTrAC - Trends in Analytical Chemistry
StatePublished - May 2012
Externally publishedYes


  • Calibration-curve method
  • Electronic referencing
  • Metabolomics
  • NMR spectroscopy
  • Nuclear magnetic resonance (NMR)
  • Pharmaceutical analysis
  • Quantitative NMR (qNMR)
  • Referencing technique
  • Standard-addition method
  • Validation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy


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