Quantitative bacteriology of endodontic infections

James Zavistoski, Jo Anne Dzink, Andrew Onderdonk, John Bartlett

Research output: Contribution to journalArticlepeer-review

48 Scopus citations


A technique is described for obtaining quantitative cultures of endodontic specimens. Paper point samplings were placed in a diluted salt solution, vortexed, and then cultured quantitatively using serial dilutions plated onto multiple media. Specimen weight was determined by weighing the point before and after specimen collection. This method was used in 10 nonvital teeth and showed a mean concentration of 107.7 bacteria per gram of sample. Nine specimens yielded a mixed aerobic-anaerobic flora, and there was an average of five different bacterial species per specimen in concentrations exceeding 105/gm.

Original languageEnglish (US)
Pages (from-to)171-174
Number of pages4
JournalOral Surgery, Oral Medicine, Oral Pathology
Issue number2
StatePublished - Feb 1980
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Dentistry(all)


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