Quantitative autoradiographic localization of NMDA, quisqualate and PCP receptors in the frog tectum

John W. McDonald; Hollis T. Cline; Martha Constantine-Paton; William F. Maragos; Michael V. Johnston; Anne B. Young

doi:10.1016/0006-8993(89)90553-2

Quantitative autoradiographic localization of NMDA, quisqualate and PCP receptors in the frog tectum

John W. McDonald
, Hollis T. Cline
, Martha Constantine-Paton
, William F. Maragos
, Michael V. Johnston
, Anne B. Young

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

An organizing role for the N-methyl-d-aspartate (NMDA) receptor/channel has been suggested in the development of the retinotectal projection in Rana pipiens. The regional distributions of NMDA, phencyclidine (PCP) and quisqualic acid (QA) receptors were quantified using in vitro autoradiography in the tectum of normal and surgically produced 3-eyed juvenile frogs. NMDA and QA receptor binding was highest in the pretectum. Of the tectal layers, the superficial retinotectal synaptic zone, layer 9, had the highest amount of NMDA and QA receptor binding. Moderate binding was observed in layer 5, with little binding in the cellular layer 6. No specific [³H]N-(1-[2-thienyl]cyclohexyl piperidine ([³H]TCP) binding was observed in any of the tectal regions.

Original language	English (US)
Pages (from-to)	155-158
Number of pages	4
Journal	Brain Research
Volume	482
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(89)90553-2
State	Published - Mar 13 1989
Externally published	Yes

Keywords

Autoradiography
Excitatory amino acid
Frog
N-methyl-d-aspartic acid
Phencyclidine
Quisqualate
Tectum
Visual plasticity

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(89)90553-2

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@article{78994e5b85684ac3baed24eef6824522,

title = "Quantitative autoradiographic localization of NMDA, quisqualate and PCP receptors in the frog tectum",

abstract = "An organizing role for the N-methyl-d-aspartate (NMDA) receptor/channel has been suggested in the development of the retinotectal projection in Rana pipiens. The regional distributions of NMDA, phencyclidine (PCP) and quisqualic acid (QA) receptors were quantified using in vitro autoradiography in the tectum of normal and surgically produced 3-eyed juvenile frogs. NMDA and QA receptor binding was highest in the pretectum. Of the tectal layers, the superficial retinotectal synaptic zone, layer 9, had the highest amount of NMDA and QA receptor binding. Moderate binding was observed in layer 5, with little binding in the cellular layer 6. No specific [3H]N-(1-[2-thienyl]cyclohexyl piperidine ([3H]TCP) binding was observed in any of the tectal regions.",

keywords = "Autoradiography, Excitatory amino acid, Frog, N-methyl-d-aspartic acid, Phencyclidine, Quisqualate, Tectum, Visual plasticity",

author = "McDonald, \{John W.\} and Cline, \{Hollis T.\} and Martha Constantine-Paton and Maragos, \{William F.\} and Johnston, \{Michael V.\} and Young, \{Anne B.\}",

note = "Funding Information: Supported by USPHS Grants 1P01NS19613 (M.V.J., A.B.Y.) MSTP Grant 5 T32 6M07863-07 J.W.M, and by N1H Grants E405818 (H.T.C.), E406039( M.C.P.).",

year = "1989",

month = mar,

day = "13",

doi = "10.1016/0006-8993(89)90553-2",

language = "English (US)",

volume = "482",

pages = "155--158",

journal = "Brain Research",

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publisher = "Elsevier BV",

number = "1",

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T1 - Quantitative autoradiographic localization of NMDA, quisqualate and PCP receptors in the frog tectum

AU - McDonald, John W.

AU - Cline, Hollis T.

AU - Constantine-Paton, Martha

AU - Maragos, William F.

AU - Johnston, Michael V.

AU - Young, Anne B.

N1 - Funding Information: Supported by USPHS Grants 1P01NS19613 (M.V.J., A.B.Y.) MSTP Grant 5 T32 6M07863-07 J.W.M, and by N1H Grants E405818 (H.T.C.), E406039( M.C.P.).

PY - 1989/3/13

Y1 - 1989/3/13

N2 - An organizing role for the N-methyl-d-aspartate (NMDA) receptor/channel has been suggested in the development of the retinotectal projection in Rana pipiens. The regional distributions of NMDA, phencyclidine (PCP) and quisqualic acid (QA) receptors were quantified using in vitro autoradiography in the tectum of normal and surgically produced 3-eyed juvenile frogs. NMDA and QA receptor binding was highest in the pretectum. Of the tectal layers, the superficial retinotectal synaptic zone, layer 9, had the highest amount of NMDA and QA receptor binding. Moderate binding was observed in layer 5, with little binding in the cellular layer 6. No specific [3H]N-(1-[2-thienyl]cyclohexyl piperidine ([3H]TCP) binding was observed in any of the tectal regions.

AB - An organizing role for the N-methyl-d-aspartate (NMDA) receptor/channel has been suggested in the development of the retinotectal projection in Rana pipiens. The regional distributions of NMDA, phencyclidine (PCP) and quisqualic acid (QA) receptors were quantified using in vitro autoradiography in the tectum of normal and surgically produced 3-eyed juvenile frogs. NMDA and QA receptor binding was highest in the pretectum. Of the tectal layers, the superficial retinotectal synaptic zone, layer 9, had the highest amount of NMDA and QA receptor binding. Moderate binding was observed in layer 5, with little binding in the cellular layer 6. No specific [3H]N-(1-[2-thienyl]cyclohexyl piperidine ([3H]TCP) binding was observed in any of the tectal regions.

KW - Autoradiography

KW - Excitatory amino acid

KW - Frog

KW - N-methyl-d-aspartic acid

KW - Phencyclidine

KW - Quisqualate

KW - Tectum

KW - Visual plasticity

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AN - SCOPUS:0024511967

SN - 0006-8993

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JO - Brain Research

JF - Brain Research

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ER -

Quantitative autoradiographic localization of NMDA, quisqualate and PCP receptors in the frog tectum

Abstract

Keywords

ASJC Scopus subject areas

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