TY - JOUR
T1 - Quantitative assessment of hemodynamic and structural characteristics of in vivo brain tissue using total diffuse reflectance spectrum measured in a non-contact fashion
AU - Song, Yinchen
AU - Garcia, Sarahy
AU - Frometa, Yisel
AU - Ramella-Roman, Jessica C.
AU - Soltani, Mohammad
AU - Almadi, Mohamed
AU - Riera, Jorge J.
AU - Lin, Wei Chiang
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Here we present a new methodology that investigates the intrinsic structural and hemodynamic characteristics of in vivo brain tissue, in a non-contact fashion, and can be easily incorporated in an intra-operative environment. Within this methodology, relative total diffuse reflectance spectra (RTD(λ)) were acquired from targets using a hybrid spectroscopy imaging system. A spectral interpretation algorithm was subsequently applied to RTD(λ) to retrieve optical properties related to the compositional and structural characteristics of each target. Estimation errors of the proposed methodology were computationally evaluated using a Monte Carlo simulation model for photon migration under various conditions. It was discovered that this new methodology could handle moderate noise and achieve very high accuracy, but only if the refractive index of the target is known. The accuracy of the technique was also validated using a series of tissue phantom studies, and consistent and accurate estimates of μs’(λ)/μa(λ) were obtained from all the phantoms tested. Finally, a smallscale animal study was conducted to demonstrate the clinical utility of the reported method, wherein a forepaw stimulation model was utilized to induce transient hemodynamic responses in somatosensory cortices. With this approach, significant stimulation-related changes (p < 0.001) in cortical hemodynamic and structural characteristics were successfully measured.
AB - Here we present a new methodology that investigates the intrinsic structural and hemodynamic characteristics of in vivo brain tissue, in a non-contact fashion, and can be easily incorporated in an intra-operative environment. Within this methodology, relative total diffuse reflectance spectra (RTD(λ)) were acquired from targets using a hybrid spectroscopy imaging system. A spectral interpretation algorithm was subsequently applied to RTD(λ) to retrieve optical properties related to the compositional and structural characteristics of each target. Estimation errors of the proposed methodology were computationally evaluated using a Monte Carlo simulation model for photon migration under various conditions. It was discovered that this new methodology could handle moderate noise and achieve very high accuracy, but only if the refractive index of the target is known. The accuracy of the technique was also validated using a series of tissue phantom studies, and consistent and accurate estimates of μs’(λ)/μa(λ) were obtained from all the phantoms tested. Finally, a smallscale animal study was conducted to demonstrate the clinical utility of the reported method, wherein a forepaw stimulation model was utilized to induce transient hemodynamic responses in somatosensory cortices. With this approach, significant stimulation-related changes (p < 0.001) in cortical hemodynamic and structural characteristics were successfully measured.
KW - Medical optics instrumentation
KW - Optical instruments
UR - http://www.scopus.com/inward/record.url?scp=85008938085&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85008938085&partnerID=8YFLogxK
U2 - 10.1364/BOE.8.000078
DO - 10.1364/BOE.8.000078
M3 - Article
C2 - 28101403
AN - SCOPUS:85008938085
SN - 2156-7085
VL - 8
SP - 78
EP - 103
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 1
M1 - #269597
ER -