TY - JOUR
T1 - Quantitative Analysis of Wound Healing after Cylindrical and Spherical Excimer Laser Ablations
AU - Shieh, Emil
AU - Moreira, Hamilton
AU - D'Arcy, John
AU - Clapham, Terrance N.
AU - McDonnell, Peter J.
PY - 1992/1/1
Y1 - 1992/1/1
N2 - Purpose: Superficial corneal ablation using the 193 nm excimer laser for the correction of myopia and astigmatism produces histologic changes in the corneal profile that can be quantitatively analyzed. Methods: Using the excimer laser, spherical and cylindrical corneal ablations were performed on rabbit eyes at depths of 2, 4, 6, and 8 diopters. After 12 weeks, the corneas were examined histologically using a dichlorotriazinyl aminofluorescein stain for collagen, and the results were quantified using a digital video image analysis system. Results: Epithelial thickness and thickness of new collagen were positively correlated with the depth of ablation after spherical ablations. With astigmatic ablations, epithelial thickness and new collagen production were correlated with ablation depth in one axis only. Conclusion: These results suggest that during cylindrical corneal ablations with the excimer laser, radially asymmetrical stromal ablations produce quantitatively asymmetrical wound healing.
AB - Purpose: Superficial corneal ablation using the 193 nm excimer laser for the correction of myopia and astigmatism produces histologic changes in the corneal profile that can be quantitatively analyzed. Methods: Using the excimer laser, spherical and cylindrical corneal ablations were performed on rabbit eyes at depths of 2, 4, 6, and 8 diopters. After 12 weeks, the corneas were examined histologically using a dichlorotriazinyl aminofluorescein stain for collagen, and the results were quantified using a digital video image analysis system. Results: Epithelial thickness and thickness of new collagen were positively correlated with the depth of ablation after spherical ablations. With astigmatic ablations, epithelial thickness and new collagen production were correlated with ablation depth in one axis only. Conclusion: These results suggest that during cylindrical corneal ablations with the excimer laser, radially asymmetrical stromal ablations produce quantitatively asymmetrical wound healing.
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U2 - 10.1016/S0161-6420(92)31851-2
DO - 10.1016/S0161-6420(92)31851-2
M3 - Article
C2 - 1495783
AN - SCOPUS:0026694228
SN - 0161-6420
VL - 99
SP - 1050
EP - 1055
JO - Ophthalmology
JF - Ophthalmology
IS - 7
ER -