Quantitative analysis of single-molecule superresolution images

Carla Coltharp, Xinxing Yang, Jie Xiao

Research output: Contribution to journalReview articlepeer-review

34 Scopus citations


This review highlights the quantitative capabilities of single-molecule localization-based superresolution imaging methods. In addition to revealing fine structural details, the molecule coordinate lists generated by these methods provide the critical ability to quantify the number, clustering, and colocalization of molecules with 10-50. nm resolution. Here we describe typical workflows and precautions for quantitative analysis of single-molecule superresolution images. These guidelines include potential pitfalls and essential control experiments, allowing critical assessment and interpretation of superresolution images.

Original languageEnglish (US)
Pages (from-to)112-121
Number of pages10
JournalCurrent Opinion in Structural Biology
Issue number1
StatePublished - Oct 2014

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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