TY - JOUR
T1 - Quantitative analysis of single-molecule superresolution images
AU - Coltharp, Carla
AU - Yang, Xinxing
AU - Xiao, Jie
N1 - Funding Information:
This work was supported by grants from the National Institutes of Health ( 1R01GM086447-01A2 ) and the National Science Foundation (EAGER MCB1019000 ).
PY - 2014/10
Y1 - 2014/10
N2 - This review highlights the quantitative capabilities of single-molecule localization-based superresolution imaging methods. In addition to revealing fine structural details, the molecule coordinate lists generated by these methods provide the critical ability to quantify the number, clustering, and colocalization of molecules with 10-50. nm resolution. Here we describe typical workflows and precautions for quantitative analysis of single-molecule superresolution images. These guidelines include potential pitfalls and essential control experiments, allowing critical assessment and interpretation of superresolution images.
AB - This review highlights the quantitative capabilities of single-molecule localization-based superresolution imaging methods. In addition to revealing fine structural details, the molecule coordinate lists generated by these methods provide the critical ability to quantify the number, clustering, and colocalization of molecules with 10-50. nm resolution. Here we describe typical workflows and precautions for quantitative analysis of single-molecule superresolution images. These guidelines include potential pitfalls and essential control experiments, allowing critical assessment and interpretation of superresolution images.
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U2 - 10.1016/j.sbi.2014.08.008
DO - 10.1016/j.sbi.2014.08.008
M3 - Review article
C2 - 25179006
AN - SCOPUS:84908686362
SN - 0959-440X
VL - 28
SP - 112
EP - 121
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
IS - 1
ER -