Quantitative analysis of cyclic AMP waves mediating aggregation in Dictyostelium discoideum

Peter N. Devreotes, Michael J. Potel, Stephen A. MacKay

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

We have previously reported the detection of cAMP waves within monolayers of aggregating Dictyostelium discoideum cells (K. J. Tomchik and P. N. Devreotes, 1981, Science 212, 443-446). The computer-assisted analysis presented here of the fluorographic images of the cAMP waves reveals (1) all the waves have a consistent width and height; (2) cAMP concentrations within centers of concentric aggregation territories oscillate periodically while at spiral centers the concentration builds up to a plateau value within 2 mm; (3) cells within the region of intersection of two oppositely directed cAMP waves are stimulated to produce more cAMP than those responding to a single wave; (4) cells start to move when the cAMP level begins to increase and cease movement when the peak cAMP concentration reaches the cell.

Original languageEnglish (US)
Pages (from-to)405-415
Number of pages11
JournalDevelopmental biology
Volume96
Issue number2
DOIs
StatePublished - Apr 1983

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Quantitative analysis of cyclic AMP waves mediating aggregation in Dictyostelium discoideum'. Together they form a unique fingerprint.

Cite this