Quantification of beading intensity in cultured neurons

R. M. Dastgheyb, G. Gallo, K. A. Barbee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present an implemented method for automated quantification of neuronal beading. Beading is the "morphological hallmark" of axonal injury and is present in many neurological disease conditions as well. In vitro models of injury result in beading which has previously been difficult to quantify, and therefore difficult to use as a measurement of the degree of axonal injury. The beading program was designed to quantify beading intensity in single axons and output a unitless beading number that represents the size and number of beads in relation to the length and width of the axon. The program was used to analyze a sample set of images and was successful in demonstrating a quantifiable difference between injured neurons and sham controls. Bland-Altman analysis was used to assess agreement between the number of beads counted by the program and those counted manually.

Original languageEnglish (US)
Title of host publication2011 IEEE 37th Annual Northeast Bioengineering Conference, NEBEC 2011
DOIs
StatePublished - Jun 16 2011
Externally publishedYes
Event37th Annual Northeast Bioengineering Conference, NEBEC 2011 - Troy, NY, United States
Duration: Apr 1 2011Apr 3 2011

Publication series

Name2011 IEEE 37th Annual Northeast Bioengineering Conference, NEBEC 2011

Other

Other37th Annual Northeast Bioengineering Conference, NEBEC 2011
Country/TerritoryUnited States
CityTroy, NY
Period4/1/114/3/11

ASJC Scopus subject areas

  • Bioengineering

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