TY - JOUR
T1 - Quantification of 3-methylglutaconic acid in urine, plasma, and amniotic fluid by isotope-dilution gas chromatography/mass spectrometry
AU - Kelley, Richard I.
N1 - Funding Information:
Supported,i n part, by grants HD-07107,H D-10981 and DK-44933 from the National institutes of Health. The technical assistanceo f Catherine Reiter is gratefullya cknowledged.
PY - 1993/11/15
Y1 - 1993/11/15
N2 - A method is described for quantification of the trace metabolite, 3-methylglutaconic acid, by isotope-dilution gas chromatography/mass spectrometry using synthetic 3-[2,4,6-13C3] methylglutaconic acid. Results are shown for quantification of 3-methylglutaconic acid in plasma, urine, cerebrospinal fluid and amniotic fluid for both normal controls and patients with different forms of 3-methylglutaconic aciduria. A simple method for the synthesis and purification of 3-[2,4,6-13C3]methylglutaconic acid is also described.
AB - A method is described for quantification of the trace metabolite, 3-methylglutaconic acid, by isotope-dilution gas chromatography/mass spectrometry using synthetic 3-[2,4,6-13C3] methylglutaconic acid. Results are shown for quantification of 3-methylglutaconic acid in plasma, urine, cerebrospinal fluid and amniotic fluid for both normal controls and patients with different forms of 3-methylglutaconic aciduria. A simple method for the synthesis and purification of 3-[2,4,6-13C3]methylglutaconic acid is also described.
KW - 3-Methylglutaconic acid
KW - Inborn error of metabolism
KW - Mass spectrometry
KW - Stable isotope dilution
UR - http://www.scopus.com/inward/record.url?scp=0027378959&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027378959&partnerID=8YFLogxK
U2 - 10.1016/0009-8981(93)90044-5
DO - 10.1016/0009-8981(93)90044-5
M3 - Article
C2 - 8111960
AN - SCOPUS:0027378959
SN - 0009-8981
VL - 220
SP - 157
EP - 164
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 2
ER -